College of Food Science and Engineering, Northwest A&F University, Yangling, China; Beijing Engineering and Technology Research Center of Food Additives, Beijing Technology & Business University (BTBU), Beijing, China.
Shaanxi Key Laboratory of Natural Products & Chemical Biology, College of Chemistry & Pharmacy, Northwest A&F University, Yangling, China.
Int J Biol Macromol. 2019 Jun 15;131:1067-1076. doi: 10.1016/j.ijbiomac.2019.03.175. Epub 2019 Mar 26.
It is still uncertain whether the consumption of Zizyphus jujuba cv. Muzao polysaccharides (ZMP) alleviates colorectal cancer (CRC) through the gut microbiota. In this study, we investigated the mortality, colon length, inflammatory factors and cecal microbiota, metabolites of healthy and azoxymethane (AOM) and dextran sodium sulfate (DSS) in induced CRC mice, consuming ZMP. Fecal-microbiota composition was examined using 16S rDNA sequencing and fecal-metabolome profiles were evaluated by ultra-high performance liquid chromatograph mass spectrometry (UHPLC-MS). The results showed ZMP consumption prevented CRC mouse colon shortening, decreased their mortality, reduced pro-inflammatory cytokines, increased the concentration of total short-chain fatty acids (SCFAs) and modulated gut microbiota in their feces. ZMP also increased the richness of Bifidobacterium, Bacteroides and Lactobacillus. In addition, among 39 perturbed metabolites, carbohydrate and amino acid production were noticeably altered. Moreover, higher correlations were found between fluctuant gut microbiota and metabolites. These findings provide mechanistic insights into the impact of dietary Zizyphus jujuba cv. Muzao polysaccharides on host health.
目前尚不清楚食用酸枣多糖（ZMP）是否通过肠道微生物群缓解结直肠癌（CRC）。在这项研究中，我们研究了食用 ZMP 的健康和氧化偶氮甲烷（AOM）和葡聚糖硫酸钠（DSS）诱导的 CRC 小鼠的死亡率、结肠长度、炎症因子和盲肠微生物群、代谢物。使用 16S rDNA 测序检查粪便微生物群组成，并通过超高效液相色谱质谱（UHPLC-MS）评估粪便代谢组图谱。结果表明，ZMP 可防止 CRC 小鼠结肠缩短，降低死亡率，减少促炎细胞因子，增加总短链脂肪酸（SCFA）的浓度，并调节粪便中的肠道微生物群。ZMP 还增加了双歧杆菌、拟杆菌和乳酸杆菌的丰富度。此外，在 39 种失调代谢物中，碳水化合物和氨基酸的产生明显改变。此外，还发现肠道微生物群和代谢物之间存在更高的相关性。这些发现为饮食中酸枣多糖对宿主健康的影响提供了机制见解。
