Richards Philip J, Connerton Phillippa L, Connerton Ian F
Division of Food Sciences, School of Biosciences, University of Nottingham, Loughborough, United Kingdom.
Front Microbiol. 2019 Mar 12;10:476. doi: 10.3389/fmicb.2019.00476. eCollection 2019.
Bacteriophage biocontrol to reduce levels in chickens can reduce human exposure and disease acquired through the consumption of contaminated poultry products. Investigating changes in the chicken microbiota during phage treatment has not previously been undertaken but is crucial to understanding the system-wide effects of such treatments to establish a sustainable application. A phage cocktail containing two virulent phages was used to treat broiler chickens colonized with HPC5. counts from cecal contents were significantly reduced throughout the experimental period but were most effective 2 days post-treatment showing a reduction of 2.4 log CFU g relative to mock-treated colonized controls. The administered phages replicated to establish stable populations. Bacteriophage predation of was not found to affect the microbiota structure but selectively reduced the relative abundance of without affecting other bacteria.
噬菌体生物防治以降低鸡体内的[具体细菌名称]水平，可减少因食用受污染家禽产品而导致的人类接触和疾病感染。此前尚未对噬菌体治疗期间鸡的微生物群变化进行研究，但这对于理解此类治疗的全系统影响以建立可持续应用至关重要。使用含有两种烈性[噬菌体名称]噬菌体的噬菌体鸡尾酒来治疗定植有[细菌名称]HPC5的肉鸡。在整个实验期间，盲肠内容物中的[细菌名称]数量显著减少，但在治疗后2天最为有效，相对于模拟治疗的定植对照，减少了2.4 log CFU/g。施用的噬菌体进行了复制以建立稳定种群。未发现噬菌体对[细菌名称]的捕食会影响微生物群结构，但选择性地降低了[细菌名称]的相对丰度，而不影响其他细菌。
