Wilson R, Pitt T, Taylor G, Watson D, MacDermot J, Sykes D, Roberts D, Cole P
J Clin Invest. 1987 Jan;79(1):221-9. doi: 10.1172/JCI112787.
Pseudomonas aeruginosa culture filtrates varied in their ability to slow human ciliary beat frequency (7-71%). This activity did not correlate with known virulence factors. However, a close correlation (r = 0.97) existed between ciliary slowing and pigment content. In a prolonged culture, the increase in activity correlated (r = 0.94) with pigment accumulation. Gel filtration of lyophilized filtrate yielded a single peak of activity corresponding to the pigment fraction. Pyocyanin extracted from an active strain, and 1-hydroxyphenazine were purified by high performance liquid chromatography, and characterized by ultraviolet absorbance spectra and mass spectrometry. Both slowed cilia in a dose-dependent manner, and were synthesized and shown to be indistinguishable from the biological compounds. Pyocyanin caused gradual onset of slowing and ultimate widespread ciliostasis with epithelial disruption. 1-hydroxyphenazine caused rapid onset of ciliary slowing associated with dyskinesia and ciliostasis. Pyocyanin assayed within filtrates accounted for a significant proportion of the bioactivity present.
铜绿假单胞菌培养滤液在减缓人类纤毛摆动频率的能力方面存在差异（7%-71%）。这种活性与已知的毒力因子无关。然而，纤毛减缓与色素含量之间存在密切相关性（r = 0.97）。在长时间培养中，活性增加与色素积累相关（r = 0.94）。对冻干滤液进行凝胶过滤产生了一个与色素部分相对应的单一活性峰。从活性菌株中提取的绿脓菌素和1-羟基吩嗪通过高效液相色谱法进行纯化，并通过紫外吸收光谱和质谱进行表征。两者均以剂量依赖性方式使纤毛减缓，并且合成后显示与生物化合物无法区分。绿脓菌素导致纤毛减缓逐渐发生，并最终导致广泛的纤毛静止和上皮破坏。1-羟基吩嗪导致纤毛快速减缓，并伴有运动障碍和纤毛静止。滤液中检测到的绿脓菌素占存在的生物活性的很大比例。
