Lin X L, White R H
J Bacteriol. 1986 Oct;168(1):444-8. doi: 10.1128/jb.168.1.444-448.1986.
Analysis of the fluorescent compounds extracted from six different species of halobacteria and one species each of Sulfolobus and Thermoplasma revealed the universal occurrence of coenzyme F420, (N-[N-[O-[5-(8-hydroxy-5-deazaisoalloxazin-10-yl)-2,3,4-trihydroxy -4-pentoxyhydroxyphosphinyl]-L-lactyl]-L-gamma-glutamyl]-L -glutamic acid), or its gamma-monoglutamyl derivative or both. The total amount (approximately 100 pmol/mg [dry weight]) of these compounds found in the halobacteria studied was approximately 5% of the amount previously reported for methanogenic bacteria. The amount of F420 found in the Sulfolobus and Thermoplasma strains was approximately 1% of that found in the halobacteria. The major compound in all but one of the examined strains was the gamma-monoglutamyl derivative of F420; one strain of halobacteria contained only F420. For the halobacterium-derived samples, the additional glutamic acid was shown to be linked by a gamma-glutamyl peptide bond to the terminal glutamic acid of the F420 core structure by enzymatic hydrolysis of the samples with three different gamma-glutamyltranspeptidases. The product of this enzymatic hydrolysis was F420 with one less glutamic acid in the side chain.
对从六种不同盐细菌以及各一种嗜热栖热菌和嗜热硫化叶菌中提取的荧光化合物进行分析后发现，辅酶F420（N-[N-[O-[5-(8-羟基-5-脱氮异咯嗪-10-基)-2,3,4-三羟基-4-戊氧基羟基膦酰基]-L-乳酰基]-L-γ-谷氨酰基]-L-谷氨酸）、其γ-单谷氨酰基衍生物或两者普遍存在。在所研究的盐细菌中发现的这些化合物总量（约100 pmol/mg[干重]）约为先前报道的产甲烷菌中该化合物量的5%。在嗜热硫化叶菌和嗜热栖热菌菌株中发现的F420量约为盐细菌中该化合物量的1%。除一个检测菌株外，所有检测菌株中的主要化合物都是F420的γ-单谷氨酰基衍生物；一个盐细菌菌株仅含有F420。对于盐细菌来源的样品，通过用三种不同的γ-谷氨酰转肽酶对样品进行酶促水解，结果表明额外的谷氨酸通过γ-谷氨酰肽键与F420核心结构的末端谷氨酸相连。这种酶促水解的产物是侧链中谷氨酸少一个的F420。
