温和热疗和高压氧通过降低脑胶质瘤中的干性增强 TMZ/PSi 纳米粒的敏感性。
Mild thermotherapy and hyperbaric oxygen enhance sensitivity of TMZ/PSi nanoparticles via decreasing the stemness in glioma.
机构信息
National Engineering Research Center for Nanomedicine, College of Life Science and Technology, Huazhong University of Science and Technology, No. 1037 Luoyu Road, Wuhan, 430074, People's Republic of China.
出版信息
J Nanobiotechnology. 2019 Apr 1;17(1):47. doi: 10.1186/s12951-019-0483-1.
BACKGROUND
Glioma is a common brain tumor with a high mortality rate. A small population of cells expressing stem-like cell markers in glioma contributes to drug resistance and tumor recurrence.
METHODS
Porous silicon nanoparticles (PSi NPs) as photothermal therapy (PTT) agents loaded with TMZ (TMZ/PSi NPs), was combined with hyperbaric oxygen (HBO) therapy in vitro and in vivo. To further investigate underlying mechanism, we detected the expression of stem-like cell markers and hypoxia related molecules in vitro and in vivo after treatment of TMZ/PSi NPs in combination with PTT and HBO.
RESULTS
NCH-421K and C6 cells were more sensitive to the combination treatment. Moreover, the expression of stem-like cell markers and hypoxia related molecules were decreased after combination treatment. The in vivo results were in line with in vitro. The combination treatment presents significant antitumor effects in mice bearing C6 tumor compared with the treatment of TMZ, PTT or TMZ/PSi NPs only.
CONCLUSION
These results suggested the TMZ/PSi NPs combined with HBO and PTT could be a potential therapeutic strategy for glioma.
背景
脑胶质瘤是一种常见的脑肿瘤，死亡率很高。脑胶质瘤中一小部分表达干细胞样细胞标志物的细胞有助于药物耐药和肿瘤复发。
方法
载有 TMZ 的多孔硅纳米粒子（TMZ/PSi NPs）作为光热治疗（PTT）剂，与高压氧（HBO）治疗联合应用于体外和体内。为了进一步研究潜在的机制，我们在体外和体内检测了 TMZ/PSi NPs 联合 PTT 和 HBO 治疗后干细胞样细胞标志物和缺氧相关分子的表达。
结果
NCH-421K 和 C6 细胞对联合治疗更敏感。此外，联合治疗后干细胞样细胞标志物和缺氧相关分子的表达降低。体内结果与体外结果一致。与 TMZ、PTT 或 TMZ/PSi NPs 单一治疗相比，该联合治疗在携带 C6 肿瘤的小鼠中表现出显著的抗肿瘤作用。
结论
这些结果表明，TMZ/PSi NPs 联合 HBO 和 PTT 可能是治疗脑胶质瘤的一种有潜力的治疗策略。
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