通过聚合酶链反应检测印度尼西亚东爪哇几个奶牛场中的凝固酶基因。
Detection of coagulase gene in from several dairy farms in East Java, Indonesia, by polymerase chain reaction.
作者信息
Effendi Mustofa Helmi, Hisyam Mirza Atikah Madarina, Hastutiek Poedji, Tyasningsih Wiwiek
机构信息
Department of Veterinary Public Health, Faculty of Veterinary Medicine, Airlangga University, Surabaya, Indonesia.
Department of Veterinary Parasitology, Faculty of Veterinary Medicine, Airlangga University, Surabaya, Indonesia.
出版信息
Vet World. 2019 Jan;12(1):68-71. doi: 10.14202/vetworld.2019.68-71. Epub 2019 Jan 10.
AIM
This study was conducted to study the coagulase () gene-based genetic diversity of , isolated from different samples of cattle from three different regions in East Java Province, Indonesia.
MATERIALS AND METHODS
A total of 160 raw milk samples collected in East Java Province, Indonesia, were screened for the presence of . The presumptive isolates were confirmed by test. The confirmed isolates were subjected to gene polymerase chain reaction.
RESULTS
Of 160 different samples, 20 (12.5%) isolates of were confirmed by positive test. Of 20 isolates, 19 (95%) isolates carried gene. Six different genotypes of gene, i.e., 440 bp, 510 bp, 547 bp, 680 bp, 740 bp, and 820 bp were obtained. One genotypes, 510 bp (10 isolates) were observed in polymorphism to be more prevalent than the others, and the genotype was present in at least one isolates from every region.
CONCLUSION
It can be concluded that gene is easily epidemiological tool for detection of variation strain from .
目的
本研究旨在研究从印度尼西亚东爪哇省三个不同地区的牛的不同样本中分离出的金黄色葡萄球菌基于凝固酶（）基因的遗传多样性。
材料与方法
对在印度尼西亚东爪哇省收集的总共160份生乳样本进行金黄色葡萄球菌检测。通过试验确认推定的分离株。对确认的金黄色葡萄球菌分离株进行基因聚合酶链反应。
结果
在160个不同样本中，通过阳性试验确认了20株（12.5%）金黄色葡萄球菌分离株。在20株金黄色葡萄球菌分离株中，19株（95%）携带基因。获得了基因的六种不同基因型，即440 bp、510 bp、547 bp、680 bp、740 bp和820 bp。观察到一种基因510 bp（10株）基因型的多态性比其他基因型更普遍，并且该基因型存在于每个地区的至少一株分离株中。
结论
可以得出结论，基因是检测金黄色葡萄球菌变异菌株的简便流行病学工具。
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