Sadri Donia, Shahsavari Fatemeh, Hezarkhani Maliheh, Shafizadeh Maryam
Dept. of Oral and Maxillofacial Pathology, Dental Branch of Tehran, Islamic Azad University, Tehran, Iran.
J Dent (Shiraz). 2019 Mar;20(1):10-15. doi: 10.30476/DENTJODS.2019.44557.
There are some differences between clinical features of central giant cell granulomas (CGCGs) and peripheral giant cell granulomas (CGCGs) despite their same microscopic features. The possible role of angiogenesis in this issue is still a matter of debate.
The aim of the present study was to compare microvessel density (MVD) between CGCGs and PGCGs of the oral cavity using CD31 and CD34.
Immunohistochemical staining was performed on 18 PGCGs and 19 CGCGs using a monoclonal antibody against CD34 and CD31. MVD was assessed and compared between the lesions using t-test for statistical analysis. < 0.05 was considered significant.
The expression levels of both CD34 and CD31 were significantly higher in CGCGs compared to PGCGs (< 0.002 and < 0.001, respectively). Significant differences in MVD assessed by both markers were observed between males and females in PGCGs (< 0.05), but not CGCGs (< 0.2).
The combined evaluation of old- and newly-formed vessels by pan-endothelial cell markers showed differences between CGCGs and PGCGs, supporting the possible vascular-proliferative nature of the former. Whether this difference has a part in their diverse biologic behaviors and the role which pre-existent vessels play in comparison to neo-formed vasculature, requires further investigation.
尽管中央巨细胞肉芽肿（CGCGs）和外周巨细胞肉芽肿（PGCGs）具有相同的微观特征，但其临床特征仍存在一些差异。血管生成在这一问题中的可能作用仍存在争议。
本研究的目的是使用CD31和CD34比较口腔CGCGs和PGCGs之间的微血管密度（MVD）。
使用抗CD34和CD31的单克隆抗体对18例PGCGs和19例CGCGs进行免疫组织化学染色。使用t检验对病变之间的MVD进行评估和比较以进行统计分析。P < 0.05被认为具有统计学意义。
与PGCGs相比，CGCGs中CD34和CD31的表达水平均显著更高（分别为P < 0.002和P < 0.001）。在PGCGs中，两种标志物评估的MVD在男性和女性之间存在显著差异（P < 0.05），但在CGCGs中没有（P < 0.2）。
通过全内皮细胞标志物对新旧血管进行联合评估显示CGCGs和PGCGs之间存在差异，支持前者可能具有血管增殖性质。这种差异是否在它们不同的生物学行为中起作用，以及与新生血管相比，既有血管所起的作用，需要进一步研究。
