O'Malley M, Pogrel M A, Stewart J C, Silva R G, Regezi J A
Department of Oral & Maxillofacial Surgery, University of California, San Francisco 94143-0424, USA.
J Oral Pathol Med. 1997 Apr;26(4):159-63. doi: 10.1111/j.1600-0714.1997.tb00451.x.
Central giant cell granulomas (CGCGs) are jaw tumors of unknown origin that often exhibit an aggressive, though unpredictable, clinical course. The purpose of this study was to determine the immunoprofile of the mononuclear cells that seem to be responsible for the biologic behavior of these tumors. Numbers of cells in cell cycle were also determined and compared in clinically aggressive and non-aggressive CGCGs. Sixteen aggressive and 12 non-aggressive CGCGs were immunohistochemically stained with antibodies to CD34, CD68, factor XIIIa, alpha-smooth muscle actin, prolyl 4-hydroxylase, Ki-67, and p53 protein. Cell populations and numbers of cells in cell cycle were determined through microscopic quantitative assessment. CD34-positive cells were limited to support vessels. CD68-positive mononuclear cells constituted a small population of cells in all tumors. With two exceptions, factor XIIIa-positive cells were rarely seen. Alpha-smooth muscle actin staining was present in approximately half the tumors, and occasionally large numbers of positive cells were seen. Most mononuclear cells were positive for fibroblast-associated antigen. No phenotypic differences were detected between aggressive and non-aggressive tumors. P53 protein did not appear to be overexpressed in CGCGs. Ki-67 staining showed that only mononuclear cells were in cell cycle, and that there were no differences between aggressive and non-aggressive tumors. We conclude that CGCGs are primarily fibroblastic (and myofibroblastic) tumors in which macrophages appear to play a secondary role. Tumor cells show no differentiation toward endothelial cells or macrophage-related dendrocytes (factor XIIIa). Cellular phenotypes and numbers of cells in cell cycle are similar in both aggressive and non-aggressive tumors.
中央巨细胞肉芽肿（CGCGs）是起源不明的颌骨肿瘤，其临床病程通常具有侵袭性，但难以预测。本研究的目的是确定似乎对这些肿瘤的生物学行为负责的单核细胞的免疫表型。还测定了临床侵袭性和非侵袭性CGCGs细胞周期中的细胞数量并进行比较。对16例侵袭性和12例非侵袭性CGCGs进行免疫组织化学染色，使用抗CD34、CD68、因子XIIIa、α-平滑肌肌动蛋白、脯氨酰4-羟化酶、Ki-67和p53蛋白的抗体。通过显微镜定量评估确定细胞群体和细胞周期中的细胞数量。CD34阳性细胞仅限于支持血管。CD68阳性单核细胞在所有肿瘤中占少数细胞群体。除两例外，很少见到因子XIIIa阳性细胞。约一半的肿瘤存在α-平滑肌肌动蛋白染色，偶尔可见大量阳性细胞。大多数单核细胞对成纤维细胞相关抗原呈阳性。侵袭性和非侵袭性肿瘤之间未检测到表型差异。CGCGs中p53蛋白似乎未过度表达。Ki-67染色显示只有单核细胞处于细胞周期，侵袭性和非侵袭性肿瘤之间无差异。我们得出结论，CGCGs主要是成纤维细胞性（和肌成纤维细胞性）肿瘤，其中巨噬细胞似乎起次要作用。肿瘤细胞未显示向内皮细胞或巨噬细胞相关树突状细胞（因子XIIIa）的分化。侵袭性和非侵袭性肿瘤的细胞表型和细胞周期中的细胞数量相似。
