Department of Medical Physics, Uppsala University Hospital, 751 85, Uppsala, Sweden.
Department of Radiology, Uppsala University Hospital, Uppsala, Sweden.
MAGMA. 2019 Aug;32(4):511-517. doi: 10.1007/s10334-019-00740-8. Epub 2019 Apr 1.
To estimate pancreas graft relaxation times and concentrations of total fat, and the intracellular lipids of non-adipose pancreatic cells (NAPC) using proton (H) magnetic resonance spectroscopy (MRS) during cold preservation.
Grafts from 11 human donors were investigated. Each pancreas was perfused in situ with histidine-tryptophan-ketoglutarate (HTK) or with University of Wisconsin solution and placed into a transport container. Temperature of the grafts was maintained at 4 ± 2 °C during transport to our hospital and MR scanning. A 1.5 T clinical scanner was used for the measurements. Single-voxel PRESS spectra were acquired using transmit-receiver head coil.
Relaxation times were measured for lipid (-CH-) (T, 287 ± 60 ms; T, 27 ± 4 ms), and tissue water (T, 670 ± 69 ms; T, 77 ± 17 ms). Average total fat, and intracellular lipids of NAPC concentrations were 79.2 ± 100.8 (range 2.4-304.4), and 2.9 ± 1.2 mmol/kg ww, respectively.
We have shown that H-MRS is a useful tool for the estimation of pancreas graft lipid concentrations. Total pancreatic fat and especially content of intracellular lipids of NAPC are valuable measures for inspection of graft quality prior to transplantation or islet of Langerhans isolation.
使用质子（H）磁共振波谱（MRS）估计冷保存过程中胰腺移植物的弛豫时间和总脂肪浓度，以及非脂肪胰腺细胞（NAPC）的细胞内脂质浓度。
对 11 位人类供体的移植物进行了研究。每个胰腺在原位用组氨酸-色氨酸-酮戊二酸（HTK）或威斯康星大学溶液灌注，然后放入运输容器中。在运往我院和磁共振扫描过程中，保持移植物的温度在 4°C±2°C。使用 1.5 T 临床扫描仪进行测量。使用发射-接收头部线圈采集单体素 PRESS 谱。
测量了脂质（-CH-）（T，287±60 ms；T，27±4 ms）和组织水（T，670±69 ms；T，77±17 ms）的弛豫时间。平均总脂肪和 NAPC 细胞内脂质浓度分别为 79.2±100.8（范围 2.4-304.4）和 2.9±1.2 mmol/kg ww。
我们已经证明，H-MRS 是估计胰腺移植物脂质浓度的有用工具。总胰腺脂肪，尤其是 NAPC 的细胞内脂质含量，是移植前或胰岛分离时检查移植物质量的有价值的指标。
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