Koburger John A, Miller Mary L
Food Science and Human Nutrition, University of Florida, Gainesville, Florida 32611.
J Food Prot. 1985 Mar;48(3):244-245. doi: 10.4315/0362-028X-48.3.244.
Application of a fluorogenic assay using 4-methylumbelliferone glucuronide (MUG) for detecting Escherichia coli in oysters was not workable due to the presence of an interfering endogenous glucuronidase in the oysters. Modification of the assay by incorporating the MUG into EC broth, rather than LST broth, eliminated the interference. When 25 oyster samples were analyzed for E. coli , 127 of the EC tubes were positive for gas. Of these, 103 were fluorescent and 102 yielded E. coli on isolation. The modification still results in saving approximately 4 d when analyzing for E. coli in oysters.
由于牡蛎中存在干扰性内源性葡萄糖醛酸酶，使用4-甲基伞形酮葡萄糖醛酸苷（MUG）的荧光测定法检测牡蛎中的大肠杆菌不可行。通过将MUG加入EC肉汤而非LST肉汤中对该测定法进行改进，消除了干扰。对25个牡蛎样本进行大肠杆菌分析时，127支EC管有产气阳性反应。其中，103支有荧光，102支在分离培养时产生了大肠杆菌。这种改进在分析牡蛎中的大肠杆菌时仍可节省约4天时间。
