Motes Miles L, Peeler James T
Fishery Research Branch, U.S. Food and Drug Administration, Dauphin Island, Alabama 36528.
Center for Food Safety and Applied Nutrition, U.S. Food and Drug Administration, Washington, DC 20204.
J Food Prot. 1991 Apr;54(4):246-248. doi: 10.4315/0362-028X-54.4.246.
Oysters and seawater collected from the southeastern United States were examined for fecal coliforms and Escherichia coli , using the current procedure of the American Public Health Association (APHA) and the fluorogenic 4-methylumbelliferyl-β-D-glucuronide (MUG) modified APHA procedure. After the presence of E. coli in both methods was confirmed by conventional IMViC procedures, there was no significant difference between method means at the α = 0.05 level. In oysters, low confirmation rates of 67 and 77% were observed by the APHA and the MUG methods, respectively. Seawater had the greatest confirmation rates (95%) by the MUG method. The MUG method may be a suitable alternative to the current APHA method for the microbiological evaluation of oysters and seawater.
采用美国公共卫生协会（APHA）的现行方法以及荧光4-甲基伞形酮基-β-D-葡萄糖醛酸苷（MUG）改良的APHA方法，对从美国东南部采集的牡蛎和海水进行了粪大肠菌群和大肠杆菌检测。在通过传统IMViC程序确认两种方法中均存在大肠杆菌后，在α = 0.05水平下，方法均值之间无显著差异。在牡蛎中，APHA方法和MUG方法的确认率分别较低，为67%和77%。海水采用MUG方法时确认率最高（95%）。MUG方法可能是当前APHA方法用于牡蛎和海水微生物学评估的合适替代方法。
