Farber Jeffrey M, Peterkin Pearl I, Sharpe Anthony N, D'Aoust Jean-Yves
Bureau of Microbial Hazards, Food Directorate, Health Protection Branch, Health and Welfare Canada, Tunney's Pasture, Ottawa, Ontario, Canada K1A 0L2.
J Food Prot. 1985 Sep;48(9):790-793. doi: 10.4315/0362-028X-48.9.790.
An enzyme-linked immunosorbent assay (ELISA) technique using a horseradish peroxidase-protein A-Spicer Edwards antiserum complex was developed for the detection of Salmonella colonies on membrane filters. In pure culture, 64 Salmonella species tested gave a positive reaction (purple stain). Of 22 naturally contaminated food samples, there was an exact correlation between the AOAC hydrophobic grid-membrane filter procedure and the ELISA technique (40.9% positives). This technique is simple, requires little equipment and can be completed in less than 2.5 h, thus allowing the detection of Salmonella spp. in foods within 48 h from initiation of sampling.
开发了一种使用辣根过氧化物酶-蛋白A-斯派塞·爱德华兹抗血清复合物的酶联免疫吸附测定（ELISA）技术，用于检测膜滤器上的沙门氏菌菌落。在纯培养中，测试的64种沙门氏菌均产生阳性反应（紫色染色）。在22个天然污染的食品样本中，AOAC疏水网格膜滤器方法与ELISA技术之间存在确切相关性（阳性率为40.9%）。该技术简单，所需设备少，可在不到2.5小时内完成，从而能够在采样开始后的48小时内检测食品中的沙门氏菌属。
