使用ISO-GRID疏水网格膜过滤器快速检测食品中的沙门氏菌属。

Rapid detection of Salmonella spp. in food by use of the ISO-GRID hydrophobic grid membrane filter.

作者信息

Entis P, Brodsky M H, Sharpe A N, Jarvis G A

出版信息

Appl Environ Microbiol. 1982 Feb;43(2):261-8. doi: 10.1128/aem.43.2.261-268.1982.

DOI:10.1128/aem.43.2.261-268.1982

PMID:7059168

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC241818/

Abstract

A rapid hydrophobic grid-membrane filter (HGMF) method was developed and compared with the Health Protection Branch cultural method for the detection of Salmonella spp. in 798 spiked samples and 265 naturally contaminated samples of food. With the HGMF method, Salmonella spp. were isolated from 618 of the spiked samples and 190 of the naturally contaminated samples. The conventional method recovered Salmonella spp. from 622 spiked samples and 204 unspiked samples. The isolation rates from Salmonella-positive samples for the two methods were not significantly different (94.6% overall for the HGMF method and 96.7% for the conventional approach), but the HGMF results were available in only 2 to 3 days after sample receipt compared with 3 to 4 days by the conventional method.

摘要

开发了一种快速疏水网格膜过滤（HGMF）方法，并将其与加拿大卫生部卫生保护局的培养方法进行比较，用于检测798份加标食品样品和265份天然污染食品样品中的沙门氏菌。采用HGMF方法，从618份加标样品和190份天然污染样品中分离出了沙门氏菌。传统方法从622份加标样品和204份未加标样品中检出了沙门氏菌。两种方法对沙门氏菌阳性样品的分离率没有显著差异（HGMF方法总体为94.6%，传统方法为96.7%），但HGMF方法在收到样品后仅2至3天即可得到结果，而传统方法则需要3至4天。