Markle R A
Proc Soc Exp Biol Med. 1986 Nov;183(2):169-76. doi: 10.3181/00379727-183-42401.
We examined specific activities (based on DNA) of six glycosidases and cathepsin C in aorta, kidney, and liver from male rats of 2, 6, 10, and 14 months of age. The premise was that assessing cellular catabolism of arterial and nonvascular tissues over age might more fully clarify the impact of age (and growth) alone upon vascular wall metabolism. All aortic glycosidases increased significantly (P less than 0.05) over the holding period as follows: neutral alpha-glucosidase, up 93%; beta-galactosidase, up 102%; N-acetyl-beta-glucosaminidase, up 119%; alpha-mannosidase, up 77%; beta-glucuronidase, up 65%; acid alpha-glucosidase, up 95%. Cathepsin C specific activity was unchanged as was aortic DNA content; total protein content increased 136%. In the kidney, all glycosidase specific activities declined over age with decreases ranging 39-55%; cathepsin C was unchanged. In the liver, neutral alpha-glucosidase increased 12%, acid alpha-glucosidase was unchanged, and the four remaining glycosidases decreased an average of 5-35% by 14 months of age. Liver cathepsin C decreased 44% over this period. Thus, enhancement of hydrolase baseline activities prevails during growth and aging in rat aortic tissue whereas hydrolases of kidney and liver tissues generally decline.
我们检测了2、6、10和14月龄雄性大鼠主动脉、肾脏和肝脏中六种糖苷酶和组织蛋白酶C的比活性（基于DNA）。前提是评估不同年龄阶段动脉组织和非血管组织的细胞分解代谢，可能会更全面地阐明年龄（和生长）本身对血管壁代谢的影响。在观察期内，所有主动脉糖苷酶均显著增加（P小于0.05），具体如下：中性α-葡萄糖苷酶增加93%；β-半乳糖苷酶增加102%；N-乙酰-β-葡萄糖胺酶增加119%；α-甘露糖苷酶增加77%；β-葡萄糖醛酸酶增加65%；酸性α-葡萄糖苷酶增加95%。组织蛋白酶C的比活性未发生变化，主动脉DNA含量也未改变；总蛋白含量增加了136%。在肾脏中，所有糖苷酶的比活性随年龄增长而下降，降幅在39%-55%之间；组织蛋白酶C未发生变化。在肝脏中，中性α-葡萄糖苷酶增加了12%，酸性α-葡萄糖苷酶未发生变化，其余四种糖苷酶在14月龄时平均下降了5%-35%。在此期间，肝脏组织蛋白酶C下降了44%。因此，在大鼠主动脉组织的生长和衰老过程中，水解酶的基线活性普遍增强，而肾脏和肝脏组织中的水解酶通常会下降。
