Markle R A
Department of Biological Sciences, Northern Arizona University, Flagstaff 86011-5640.
Exp Mol Pathol. 1989 Aug;51(1):9-17. doi: 10.1016/0014-4800(89)90003-8.
Normal arterial foci which take up Evans blue dye (EBD) in vivo are believed to represent atherosclerosis-prone, hemodynamically stressed foci compared to areas which exclude dye. We have used the rabbit EBD model to examine focal aortic hydrolases of blue areas versus white areas, and we report herein significant focal variations of hydrolase activities. Enzymes measured included neutral alpha-glucosidase, N-acetyl-beta-glucosaminidase, alpha-mannosidase, acid alpha-glucosidase, beta-galactosidase, beta-glucuronidase, cathepsin C, and acid cholesteryl esterase (ACE); specific activities were expressed on the basis of tissue DNA. In correlative areas of EBD uptake in normal rabbit aortic arch, ACE activity averaged 17% higher and cathepsin C activity averaged 37% lower than activities of areas free of EBD in the descending thoracic aorta (P less than 0.02). None of the glycosidases studied differed significantly between blue and white aortic areas. These findings indicate that discrete, intrinsic differences of hydrolytic enzyme activities exist in the normal rabbit aorta in areas delineated by in vivo EBD uptake, areas recognized as lesion-prone vs lesion-resistant.
在体内摄取伊文思蓝染料（EBD）的正常动脉病灶，与不摄取染料的区域相比，被认为代表了易发生动脉粥样硬化、承受血流动力学压力的病灶。我们使用兔EBD模型来检测蓝色区域与白色区域的主动脉局部水解酶，并且在此报告水解酶活性存在显著的局部差异。所检测的酶包括中性α-葡萄糖苷酶、N-乙酰-β-葡萄糖胺酶、α-甘露糖苷酶、酸性α-葡萄糖苷酶、β-半乳糖苷酶、β-葡萄糖醛酸酶、组织蛋白酶C和酸性胆固醇酯酶（ACE）；比活性以组织DNA为基础表示。在正常兔主动脉弓中摄取EBD的相关区域，ACE活性平均比胸降主动脉中未摄取EBD区域的活性高17%，组织蛋白酶C活性平均低37%（P<0.02）。所研究的糖苷酶在主动脉的蓝色和白色区域之间均无显著差异。这些发现表明，在正常兔主动脉中，通过体内EBD摄取所界定的区域，即被认为易患病变与抗病变的区域，存在水解酶活性的离散性、内在差异。
