Grupo de Marcadores Inmunológicos, Laboratorio de Genética e Inmunología Molecular, Instituto de Biología, Facultad de Ciencias, Pontificia Universidad Católica de Valparaíso, Chile.
Laboratorio de Biotecnología Acuática y Genómica Reproductiva, Instituto de Biología, Facultad de Ciencias, Universidad de Valparaíso, Chile.
Fish Shellfish Immunol. 2019 Jun;89:505-515. doi: 10.1016/j.fsi.2019.03.069. Epub 2019 Mar 30.
The scallop Argopecten purpuratus is one of the most economically important cultured mollusks on the coasts from Chile and Peru but its production has declined, in part, due to the emergence of mass mortality events of unknown origin. Driven by this scenario, increasing progress has been made in recent years in the comprehension of immune response mechanisms in this species. However, it is still not entirely understood how different mucosal interfaces participate and cooperate with the immune competent cells, the hemocytes, in the immune defense. Thus, in this work we aimed to characterize the transcriptome of three tissues with immune relevance from A. purpuratus by next-generation sequencing and de novo transcriptome assembly. For this, 18 cDNA libraries were constructed from digestive gland, gills and hemocytes tissues of scallops from different immune conditions and sequenced by the Illumina HiSeq4000 platform. A total of 967.964.884 raw reads were obtained and 967.432.652 clean reads were generated. The clean reads were de novo assembled into 46.601 high quality contigs and 32.299 (69.31%) contigs were subsequently annotated. In addition, three de novo specific assemblies were performed from clean reads obtained from each tissue cDNA libraries for their comparison. Gene ontology (GO) and KEGG analyses revealed that annotated sequences from digestive gland, gills and hemocytes could be classified into both general and specific subcategory terms and known biological pathways, respectively, according to the tissue nature. Finally, several immune related candidate genes were identified, and the differential expression of tissue-specific genes was established, suggesting they could display specific roles in the host defense. The data presented in this study provide the first insight into the tissue specific transcriptome profiles of A. purpuratus, which should be considered for further research on the interplay between the hemocytes and mucosal immune responses.
扇贝多肽 Argopecten purpuratus 是智利和秘鲁沿海地区经济最重要的养殖贝类之一，但由于未知原因的大规模死亡事件的出现，其产量有所下降。受此影响，近年来人们对该物种免疫反应机制的理解取得了越来越多的进展。然而，不同黏膜界面如何与具有免疫功能的细胞——血细胞相互作用和合作，以进行免疫防御，仍然不完全清楚。因此，在这项工作中，我们旨在通过下一代测序和从头转录组组装来描述 A. purpuratus 三种具有免疫相关性的组织的转录组。为此，我们从不同免疫状态的扇贝多肽的消化腺、鳃和血细胞组织构建了 18 个 cDNA 文库，并通过 Illumina HiSeq4000 平台进行测序。共获得 967964884 条原始读数，生成 967432652 条清洁读数。将清洁读数从头组装成 46601 条高质量的连续序列，其中 32299 条（69.31%）连续序列随后被注释。此外，还从每个组织 cDNA 文库的清洁读数中进行了三次从头特定组装，以进行比较。GO 和 KEGG 分析表明，根据组织性质，消化腺、鳃和血细胞的注释序列可分为一般和特定亚类术语和已知的生物学途径。最后，鉴定了几个免疫相关的候选基因，并确定了组织特异性基因的差异表达，这表明它们在宿主防御中可能具有特定的作用。本研究提供了 A. purpuratus 组织特异性转录组图谱的第一个见解，这应该被认为是进一步研究血细胞和黏膜免疫反应之间相互作用的基础。
