Food Engineering & Machinery Group, School of Mechanical Engineering, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, Jiangsu, China.
Jiangsu Key Laboratory of Advanced Food Manufacturing Equipment & Technology, 1800 Lihu Avenue, Wuxi 214122, Jiangsu, China.
Molecules. 2017 Nov 21;22(11):2023. doi: 10.3390/molecules22112023.
Cell disruption is an important step during the extraction of C-phycocyanin from . An ultrafine shearing method is introduced and combined with soaking and ultrasonication to disrupt the cell walls of efficiently and economically. Five kinds of cell disruption method, including soaking, ultrasonication, freezing-thawing, soaking-ultrafine shearing and soaking-ultrafine shearing-ultrasonication were applied to break the cell walls of . The effectiveness of cell breaking was evaluated based on the yield of the C-phycocyanin. The results show that the maximum C-phycocyanin yield was 9.02%, achieved by the soaking-ultrafine shearing-ultrasonication method, followed by soaking (8.43%), soaking-ultrafine shearing (8.89%), freezing and thawing (8.34%), and soaking-ultrasonication (8.62%). The soaking-ultrafine shearing-ultrasonication method is a novel technique for breaking the cell walls of for the extraction of C-phycocyanin.
细胞破碎是从. 中提取 C-藻蓝蛋白的重要步骤。介绍了一种超微剪切方法，并将其与浸泡和超声处理相结合，以高效、经济地破坏. 的细胞壁。五种细胞破碎方法，包括浸泡、超声处理、冻融、浸泡-超微剪切和浸泡-超微剪切-超声处理，用于破坏. 的细胞壁。根据 C-藻蓝蛋白的产量来评估细胞破碎的效果。结果表明，通过浸泡-超微剪切-超声处理法获得的 C-藻蓝蛋白最大产量为 9.02%，其次是浸泡法（8.43%）、浸泡-超微剪切法（8.89%）、冻融法（8.34%）和浸泡-超声处理法（8.62%）。浸泡-超微剪切-超声处理法是一种新型的破坏. 细胞壁的技术，用于提取 C-藻蓝蛋白。
