Tikhonova O V, Zaslavskaia P L, Lur'e L M, Bartoshevich Iu E
Antibiot Med Biotekhnol. 1986 Jul;31(7):514-9.
When grown on media with vegetable oils the producer of penicillin was more sensitive to changes in the conditions of mass transfer. Their worsening resulted in a more significant decrease in the level of the mycelium productivity than that of media containing animal fats. The observed differences were associated with the fact that the rate of consumption of readily assimilated fat substrates, for instance sunflower oil, by the cells was sufficiently high even at defective aeration and agitation. Their further oxidation in the mycelium was retarded which resulted in increasing lipid levels in the cells, irreversible destruction of the mitochondria, followed by further lowering of the oxidation processes in the cells, and inhibition of basic and secondary metabolism. On media containing whale oil there were also changes in the structure of the hyphae and first of all mitochondria of Penicillium chrysogenum and inhibited oxidation of fats evident from fractional composition of the mycelium lipids. However, all these changes were less pronounced because of the low rate of the whale oil assimilation by the cells.
当在含有植物油的培养基上生长时，青霉素生产菌对传质条件的变化更为敏感。与含有动物脂肪的培养基相比，传质条件变差会导致菌丝体生产力水平下降得更为显著。观察到的差异与以下事实有关：即使在通气和搅拌不良的情况下，细胞对易于同化的脂肪底物（例如向日葵油）的消耗速率也足够高。它们在菌丝体中的进一步氧化受到阻碍，这导致细胞内脂质水平升高、线粒体不可逆破坏，进而导致细胞内氧化过程进一步降低，并抑制初级和次级代谢。在含有鲸油的培养基上，产黄青霉的菌丝结构，尤其是线粒体结构也发生了变化，并且从菌丝体脂质的馏分组成可以明显看出脂肪氧化受到抑制。然而，由于细胞对鲸油的同化速率较低，所有这些变化都不太明显。
