Abdollahzadeh Estakhri Mohammadreza, Shokrzadeh Mohammad, Jaafari Mahmoud Reza, Karami Mohammad, Mohammadi Hamidreza
Pharmaceutical Sciences Research Center, Faculty of Pharmacy and Student Research Committee, Mazandaran University of Medical Sciences, Sari, Iran.
Department of Toxicology and Pharmacology, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran.
Iran J Basic Med Sci. 2019 Jan;22(1):17-24. doi: 10.22038/ijbms.2018.23229.5874.
Diazinon (DZ) is an organophosphate pesticide that induces oxidative damage in different organs. The aim of this study was to compare the effectiveness of nanomicelles containing curcuminoids (NCUR) and natural curcumin (CUR) in attenuating the oxidative damage induced by DZ in male rats.
After a single intraperitoneal (IP) injection of DZ (100 mg/kg), the rats were administered either CUR or NCUR (25 and 60 mg/kg, IP). Biomarkers of cell damage including, alanine transaminase (ALT), aspartate transaminase (AST), alkaline phosphatase (ALP), creatinine (Cr), urea, lactate dehydrogenase (LDH), creatine kinase-MB isoenzyme (CK-MB) and troponin I, were quantified in the serum. Lipid peroxidation (LPO) and glutathione (GSH) content in the liver, kidney, and heart tissues were determined.
DZ administration increased the serum levels of ALT, AST, ALP, Cr, urea, LDH, CK-MB, and troponin I; however, the levels significantly (<0.001) decreased in the CUR- and NCUR-treated groups compared to those in the DZ group. NCUR significantly decreased LPO (<0.05) and increased GSH (<0.05) in the heart, kidney, and liver tissues at all doses (especially, at 60 mg/kg) compared with CUR.
Our findings suggest that NCUR treatment counters DZ-induced oxidative tissue damage to a greater extent than CUR.
二嗪农（DZ）是一种有机磷农药，可在不同器官中诱导氧化损伤。本研究的目的是比较含有姜黄素类化合物的纳米胶束（NCUR）和天然姜黄素（CUR）减轻DZ诱导的雄性大鼠氧化损伤的效果。
单次腹腔注射DZ（100mg/kg）后，给大鼠腹腔注射CUR或NCUR（25mg/kg和60mg/kg）。对血清中包括丙氨酸转氨酶（ALT）、天冬氨酸转氨酶（AST）、碱性磷酸酶（ALP）、肌酐（Cr）、尿素、乳酸脱氢酶（LDH）、肌酸激酶-MB同工酶（CK-MB）和肌钙蛋白I在内的细胞损伤生物标志物进行定量分析。测定肝脏、肾脏和心脏组织中的脂质过氧化（LPO）和谷胱甘肽（GSH）含量。
给予DZ后，血清中ALT、AST、ALP、Cr、尿素、LDH、CK-MB和肌钙蛋白I水平升高；然而，与DZ组相比，CUR和NCUR治疗组的这些水平显著降低（<0.001）。与CUR相比，所有剂量（尤其是60mg/kg）的NCUR均能显著降低心脏、肾脏和肝脏组织中的LPO（<0.05）并增加GSH（<0.05）。
我们的研究结果表明，NCUR治疗比CUR更能有效对抗DZ诱导的氧化组织损伤。
