Molecular epidemiology of colistin-resistant producing NDM-1 from hospitalized patients in Iran.

OBJECTIVES

Resistance to carbapenems is the principal reason for the continuing utilization of colistin as a last resort choice for treating the infections resulted from multidrug carbapenem-resistant (CRPA) isolates. The assessment of antimicrobial resistance pattern, the prevalence of carbapenem-resistance determinants, and molecular epidemiology of colistin-resistant isolates among CRPA strains were the aims of the present research.

MATERIALS AND METHODS

The current cross-sectional research was conducted on 269 CRPA isolates collected from various clinical samples from 2013 to 2016. After performing identification tests, disk diffusion as well as MIC methods were used for testing sensitivity to the antibiotics. Modified Hodge Test (MHT) was utilized to produce carbapenemase. PCR technique identified beta-lactamase classes A, B, and D genes.

RESULTS

In total, from 269 CRPA, five isolates (1.3%) were resistant to colistin. It was found that , and genes were present in 40%, 40%, 20%, and 100% of colistin-resistant isolates, respectively. DLST type 25-11 is a significant cluster of colistin-resistant isolates.

CONCLUSION

The appearance of colistin-resistant isolates in CRPA carrying NDM-1 with multiple carbapenem-resistant genes shows the great problem in the treatment of infections.