Irstea Lyon, Riverly Research Unit, Villeurbanne, France.
Grupo de Biología y Toxicología Ambiental, Facultad de Ciencias, Universidad Nacional de Educación a Distancia (UNED), Madrid, Spain.
Sci Total Environ. 2019 Jun 25;671:874-882. doi: 10.1016/j.scitotenv.2019.03.384. Epub 2019 Mar 27.
The toxicity of three field-collected sediments differentially contaminated with pesticides, heavy metals, phtalates and polycyclic aromatic hydrocarbons (PAHs), was assessed in Chironomus riparius. For this purpose, C. riparius larvae were exposed throughout their entire life cycle to sediments collected in three sites along the Saulx river in France, and the toxic effects were measured at different levels of biological organization: from the molecular (lipidomic analysis and transcriptional variations) to the whole organism response (respiration rate, shape markers and emergence rate). In the sediment characterized by an intermediate level of contamination with PAHs and phtalates, we detected an increase of the cell stress response and delayed emergence of males. In the group exposed to the most contaminated sediment with PAHs, phtalates and pesticides, genes related to endocrine pathways, cell stress response and biotransformation processes were overexpressed, while female wing shape was affected. Field-collected sediment exposure did not induce significant effects on mentum shape markers or on the lipid profile. The present study provides new insights into the multilevel effects of differentially contaminated sediments in insects. This integrative approach will certainly contribute to improved assessment of the risk that complex mixtures of pollutants pose to the aquatic ecosystem.
本研究评估了三种野外采集的沉积物的毒性，这些沉积物分别受到农药、重金属、邻苯二甲酸酯和多环芳烃（PAHs）的不同程度污染。为此，我们将摇蚊幼虫的整个生命周期暴露于法国 Saulx 河沿岸的三个地点采集的沉积物中，并在不同的生物组织水平上测量了毒性效应：从分子水平（脂质组分析和转录变化）到整个生物体反应（呼吸速率、形态标记和成虫出现率）。在 PAHs 和邻苯二甲酸酯污染程度中等的沉积物中，我们检测到细胞应激反应增加和雄性成虫出现延迟。在暴露于 PAHs、邻苯二甲酸酯和农药污染最严重的沉积物组中，与内分泌途径、细胞应激反应和生物转化过程相关的基因表达上调，而雌性翅膀形状受到影响。野外采集的沉积物暴露对下颚形态标记或脂质谱没有引起显著影响。本研究为昆虫中不同程度污染沉积物的多层次效应提供了新的见解。这种综合方法无疑将有助于改善对复杂污染物混合物对水生生态系统构成的风险评估。
