Jin Yuan-Zhe, Zheng Guang-Bin, Lee Jae Hyup
a Department of Orthopedic Surgery, College of Medicine, Seoul National University , Seoul , South Korea.
b Department of Orthopaedics, Taizhou Hospial of Zhejiang Province , Zhejiang , China.
Growth Factors. 2019 Apr;37(1-2):85-94. doi: 10.1080/08977194.2019.1596905. Epub 2019 Apr 4.
bone morphogenetic protein-2 (ErhBMP-2) had a larger yield but less osteoinductivity than Chinese hamster ovary cell bone morphogenetic protein-2 (CrhBMP-2). Since the release profile of rhBMP-2 affects its osteoinductivity, an appropriate carrier could improve the effect of ErhBMP-2. Demineralized bone matrix (DBM) was one of the most widely used bone substitutes, but few studies evaluated the osteoinductivity of ErhBMP-2 while it was carried by DBM. Therefore, we compared the osteoinductivity of ErhBMP-2 with CrhBMP-2 with DBM as the carrier of each. results showed ErhBMP-2 had slightly less osteoinductivity than CrhBMP-2. However, with DBM as the carrier, ErhBMP-2 induced significantly more bone regeneration in rat calvaria defects. Therefore, ErhBMP-2 might have comparable osteoinductivity with CrhBMP-2 while carried by DBM.
重组人骨形态发生蛋白-2（ErhBMP-2）的产量较高，但与中国仓鼠卵巢细胞骨形态发生蛋白-2（CrhBMP-2）相比，其骨诱导活性较低。由于重组人骨形态发生蛋白-2的释放曲线会影响其骨诱导活性，因此合适的载体可以提高ErhBMP-2的效果。脱矿骨基质（DBM）是使用最广泛的骨替代物之一，但很少有研究评估DBM携带ErhBMP-2时的骨诱导活性。因此，我们将DBM作为各自的载体，比较了ErhBMP-2和CrhBMP-2的骨诱导活性。结果显示，ErhBMP-2的骨诱导活性略低于CrhBMP-2。然而，以DBM作为载体时，ErhBMP-2在大鼠颅骨缺损中诱导的骨再生明显更多。因此，当由DBM携带时，ErhBMP-2可能具有与CrhBMP-2相当的骨诱导活性。
