Sakai K, Luppi P H, Salvert D, Kimura H, Maeda T, Jouvet M
C R Acad Sci III. 1986;303(8):317-24.
The localization of cholinergic neurons in the cat lower brain stem was determined immunocytochemically with a monoclonal antibody against choline acetyltransferase (ChAT), the acetylcholine synthesizing enzyme. ChAT-positive neurons were observed in four major cell groups: cranial nerve motor and special visceromotor neurons: parasympathetic preganglionic visceromotor neurons; neurons located in the ponto-mesencephalic tegmentum including area X (or pedunculopontine tegmental nucleus), nucleus laterodorsalis tegmenti (Ldt) of Castaldi, and peri-locus coeruleus alpha (peri-alpha); and neurons located in nucleus reticularis magnocellularis (Mc) and adjacent nucleus reticularis gigantocellularis (Gc) of the medulla.
利用针对胆碱乙酰转移酶(ChAT,乙酰胆碱合成酶)的单克隆抗体,通过免疫细胞化学方法确定了猫脑桥下部胆碱能神经元的定位。在四个主要细胞群中观察到ChAT阳性神经元:脑神经运动和特殊内脏运动神经元;副交感神经节前内脏运动神经元;位于脑桥中脑被盖的神经元,包括X区(或脚桥被盖核)、卡斯塔尔迪背外侧被盖核(Ldt)和蓝斑周α区(周α区);以及位于延髓巨细胞网状核(Mc)和相邻的巨细胞网状核(Gc)中的神经元。