Donahoe R M, Nicholson J K, Madden J J, Donahoe F, Shafer D A, Gordon D, Bokos P, Falek A
Clin Immunol Immunopathol. 1986 Nov;41(2):254-64. doi: 10.1016/0090-1229(86)90109-1.
Simultaneous and independent use of cocaine and alcohol by heroin addicts was shown to variably modulate the ability of their T cells to form E-rosettes with sheep erythrocytes (E). As reported previously, the percentages of E-rosette-forming T cells of both active and total types were depressed in association with heroin addiction. We show here that the kinetic curve of the rate of E-rosette formation is also depressed by heroin use and that the use of cocaine but not alcohol by heroin addicts reverses depression of E-rosette formation by heroin. The percentages of E-rosette-forming T cells from the bloods of heroin addicts who used both alcohol and cocaine, as well as the kinetic rate curves of E-rosette formation, were intermediate between the essentially normal levels found for heroin addicts who used cocaine and the severely depressed levels evident for users of heroin alone or heroin plus alcohol. Modulation of the levels of E-rosette formation by alcohol used in conjunction with cocaine and/or heroin was variably dose dependent. Polydrug effects evident by analyses of E-rosette formation were not seen when the percentages of lymphocytes reactive with LYT-3 (anti-E-receptor, 9.6 epitope) and OKT-3 (anti-total T cell) monoclonal antibodies were assessed cytofluorometrically, although the data suggested that subnormal percentages of LYT-3+ T cells were present when heroin addicts also used cocaine. These findings are relevant to basic understanding of T-cell physiology from a neuroimmunological perspective and also suggest ways that addictive drugs may modulate the immunocompetence of drug addicts.
海洛因成瘾者同时且独立使用可卡因和酒精，结果显示其T细胞与绵羊红细胞（E）形成E花环的能力受到不同程度的调节。如先前报道，无论是活跃型还是总类型的E花环形成T细胞百分比，在海洛因成瘾时都会降低。我们在此表明，海洛因使用也会降低E花环形成速率的动力学曲线，并且海洛因成瘾者使用可卡因而非酒精可逆转海洛因对E花环形成的抑制作用。同时使用酒精和可卡因的海洛因成瘾者血液中E花环形成T细胞的百分比，以及E花环形成的动力学速率曲线，介于使用可卡因的海洛因成瘾者基本正常的水平和仅使用海洛因或海洛因加酒精的使用者明显严重降低的水平之间。与可卡因和/或海洛因联合使用的酒精对E花环形成水平的调节在不同程度上呈剂量依赖性。当通过细胞荧光法评估与LYT - 3（抗E受体，9.6表位）和OKT - 3（抗总T细胞）单克隆抗体反应的淋巴细胞百分比时，未观察到通过E花环形成分析明显的多药效应，尽管数据表明海洛因成瘾者同时使用可卡因时，LYT - 3 + T细胞百分比低于正常水平。这些发现从神经免疫学角度对于深入理解T细胞生理学具有重要意义，也提示了成瘾药物可能调节吸毒者免疫能力的方式。
