A novel human lncRNA SANT1 -regulates the expression of by altering SFPQ/E2F1/HDAC1 binding to the promoter region in renal cell carcinoma.

encodes MATE 2-K in the kidney, which mediates the secretion of certain endogenous and exogenous compounds. was dramatically repressed in patients with renal cell carcinoma (RCC), and a lower level of might act as a negative prognostic marker, although the mechanism is not well understood. In this study, we aimed to investigate the mechanism which is downregulated in RCC. Based on the annotation information of the locus available in the UCSC genome browser database, we identified a novel lncRNA, which is transcribed from the locus and named it SANT1. Overexpression and knock-down assays were performed to investigate the effects of SANT1 on -regulation of . We verified the direct binding between SANT1 and SFPQ/E2F1/HDAC1 using the cross-linking and immunoprecipitation (CLIP) assay. Chromatin immunoprecipitation was performed to confirm the molecular mechanism which SANT1 activates the transcription of the coding region. We observed that SANT1 can -regulate its own genetic locus. In tumour-adjacent tissues, the locus highly expresses SANT1, which can remove the regulatory SFPQ/E2F1/HDAC1 suppressor complex from the promoter region, thereby significantly increasing the levels of the H3K27ac modification and RNAPII binding. Owing to a low SANT1 level, the binding of this inhibitory complex in the promoter region is upregulated in RCC, which results in silencing of the coding region. In conclusion, we identified a novel lncRNA and elucidated the mechanism which it regulates expression in RCC.