Minerva Foundation Institute for Medical Research, Biomedicum Helsinki 2U, Tukholmankatu 8, 00290 Helsinki, Finland.
HiLIFE - Neuroscience Center, University of Helsinki, Haartmaninkatu 8, 00290 Helsinki, Finland.
Biol Chem. 2019 Aug 27;400(9):1141-1146. doi: 10.1515/hsz-2019-0111.
The axon initial segment (AIS) comprises a sub-membranous lattice containing periodic actin rings. The overall AIS structure is insensitive to actin-disrupting drugs, but the effects of actin-disrupting drugs on actin rings lack consensus. We examined the effect of latrunculin A and B on the actin cytoskeleton of neurons in culture and actin rings in the AIS. Both latrunculin A and B markedly reduced the overall amount of F-actin in treated neurons in a dose-dependent manner, but the periodicity of actin rings remained unaffected. The insensitivity of AIS actin rings to latrunculin suggests they are relatively stable.
轴突起始段（AIS）包含一个亚膜晶格，其中包含周期性的肌动蛋白环。AIS 的整体结构对肌动蛋白破坏药物不敏感，但肌动蛋白破坏药物对肌动蛋白环的影响缺乏共识。我们研究了拉曲库林 A 和 B 对培养神经元中的肌动蛋白细胞骨架和 AIS 中肌动蛋白环的影响。拉曲库林 A 和 B 均以剂量依赖性方式显著降低处理神经元中总的 F-肌动蛋白含量，但肌动蛋白环的周期性不受影响。AIS 肌动蛋白环对拉曲库林不敏感表明它们相对稳定。
