Department of Andrology, Shenzhen Traditional Chinese Medicine Hospital, Shenzhen, 518033, Guangdong, China.
Department of Andrology, Shenzhen Baoan Traditional Chinese Medicine Hospital, Shenzhen, 518133, Guangdong, China.
Biomed Pharmacother. 2019 Jun;114:108822. doi: 10.1016/j.biopha.2019.108822. Epub 2019 Apr 2.
In this work, we evaluated the expression of microRNA-384 (miR-384) in human prostate cancer (CAP) cells and its regulatory role on CAP in vitro and in vivo functions.
In CAP cell lines, miR-384 expression was evaluated by qRT-PCR. In LNCaP and VCaP cells, lentiviral infection was done to overexpress miR-384. The regulatory effects of miR-384 overexpression on CAP in vitro proliferation and migration, and in vivo tumorigenesis, were evaluated, respectively. The targeting of miR-384 on Homeobox b7 gene (HOXB7), was evaluated by dual-luciferase reporter assay and qRT-PCR. In addition, HOXB7 was upregulated in miR-384-overexpressed CAP cells to evaluate the correlated role of HOXB7 in miR-384-mediated CAP proliferation and migration in vitro.
MiR-384 was lowly expressed in CAP cell lines. Lentiviral infection induced miR-384 overexpression inhibited CAP in vitro proliferation and migration, and in vivo tumorigenesis. HOXB7 was directly targeted by miR-384 in CAP cells. In miR-384-overexpressed CAP cells, HOXB7 upregulation reversed the effect of miR-384 by promoting CAP in vitro proliferation and migration.
MiR-384 was downregulated in CAP cells. MiR-384 overexpression suppressed CAP in vitro and in vivo development, possibly via acting through its downstream target gene of HOXB7.
在这项工作中，我们评估了 microRNA-384（miR-384）在人前列腺癌（CAP）细胞中的表达及其对 CAP 的体外和体内功能的调节作用。
在 CAP 细胞系中，通过 qRT-PCR 评估 miR-384 的表达。在 LNCaP 和 VCaP 细胞中，通过慢病毒感染过表达 miR-384。分别评估 miR-384 过表达对 CAP 体外增殖和迁移以及体内肿瘤发生的调节作用。通过双荧光素酶报告基因检测和 qRT-PCR 评估 miR-384 对同源盒 B7 基因（HOXB7）的靶向作用。此外，在 miR-384 过表达的 CAP 细胞中上调 HOXB7，以评估 HOXB7 在 miR-384 介导的 CAP 体外增殖和迁移中的相关作用。
miR-384 在 CAP 细胞系中低表达。慢病毒感染诱导 miR-384 过表达抑制 CAP 体外增殖和迁移以及体内肿瘤发生。HOXB7 是 CAP 细胞中 miR-384 的直接靶标。在 miR-384 过表达的 CAP 细胞中，HOXB7 的上调通过促进 CAP 体外增殖和迁移来逆转 miR-384 的作用。
miR-384 在 CAP 细胞中下调。miR-384 过表达抑制 CAP 的体外和体内发育，可能是通过其下游靶基因 HOXB7 发挥作用。
