Department of Chemistry, Lanzhou University, Lanzhou 730000, PR China.
Department of Chemistry, Lanzhou University, Lanzhou 730000, PR China.
Talanta. 2019 May 15;197:204-210. doi: 10.1016/j.talanta.2019.01.022. Epub 2019 Jan 8.
A new fluorescent probe was designed and synthesized according to the photo-induced electron transfer theory (PET) and the results of density functional theory calculation. The synthesized probe had a larger Stokes shift (130 nm) and double emission peak in response to highly toxic thiophenols, which could be applied in rapid and high sensitive detection of thiophenol in aqueous solution. A 25-fold fluorescence intensity enhancement was achieved and the fluorescent intensity at 460 nm had a linear relationship to the thiophenol in concentration range of 0-2.0 μM. Furthermore, a remarkable detection limit (6 nM) could be achieved. 50-fold other species have no interference to the detection. The practical utility of the probe was demonstrated by live HeLa cells imaging and thiophenol detection in real water samples. In linear range of 0-2.0 μM, the probe showed good recoveries (from 95% to 108%) for thiophenol detection in natural water samples. This method possesses high sensitivity, lower detection limit and fast response (i.e., 10 min, which is shorter than the average time response of other reported methods), indicating that the synthesized probe would be promising for real-time detection of thiophenols.
根据光诱导电子转移理论（PET）和密度泛函理论计算的结果，设计并合成了一种新的荧光探针。合成的探针具有较大的斯托克斯位移（130nm）和双发射峰，对高毒性的硫酚有响应，可应用于水溶液中硫酚的快速高灵敏检测。荧光强度增强了 25 倍，在 460nm 处的荧光强度与硫酚的浓度在 0-2.0μM 范围内呈线性关系。此外，还可以达到显著的检测限（6nM）。50 倍的其他物质对检测没有干扰。通过活 HeLa 细胞成像和实际水样中的硫酚检测，证明了探针的实际应用。在 0-2.0μM 的线性范围内，该探针对天然水样中硫酚的检测具有良好的回收率（95%-108%）。该方法具有灵敏度高、检测限低、响应速度快（即 10min，比其他报道方法的平均响应时间短）的特点，表明合成的探针有望用于实时检测硫酚。
