在酸性甲醇中加热细胞30分钟而不进行冷冻干燥，可为分枝杆菌的气相色谱表征提供足够产量的脂肪酸和醇类。
Heating cells in acid methanol for 30 min without freeze-drying provides adequate yields of fatty acids and alcohols for gas chromatographic characterization of mycobacteria.
作者信息
Jimenez J, Larsson L
出版信息
J Clin Microbiol. 1986 Nov;24(5):844-5. doi: 10.1128/jcm.24.5.844-845.1986.
Abstract
We studied the release of mycobacterial fatty acids (as methyl esters) and secondary alcohols after heating both wet and freeze-dried cells in methanolic hydrogen chloride for different time periods. A 30-min heating of the mycobacteria without prior freeze-drying was found adequate in a routine gas chromatographic procedure for strain and species characterization.
摘要
我们研究了在甲醇盐酸中对湿细胞和冻干细胞加热不同时间段后，分枝杆菌脂肪酸（以甲酯形式）和仲醇的释放情况。发现在用于菌株和菌种鉴定的常规气相色谱程序中，分枝杆菌在未预先冻干的情况下加热30分钟就足够了。
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