College of Food Science & Institute of Food Biotechnology, South China Agricultural University, Guangzhou, 510640, China; Research Center for Micro-Ecological Agent Engineering and Technology of Guangdong Province, Guangzhou, 510640, China.
College of Food Science & Institute of Food Biotechnology, South China Agricultural University, Guangzhou, 510640, China.
Microb Pathog. 2019 Jun;131:205-211. doi: 10.1016/j.micpath.2019.04.003. Epub 2019 Apr 3.
Musa basjoo is a kind of popular slimming fruit in southern China. However, even though the trophic component and physiological effect are well studied, its internal mechanism in reconstructing gut microbiota remains unclear. In this study, maturity of M. basjoo were divided into four levels. Results indicated that M. basjoo in level Ⅱ (with 35% maturity) represented the greatest increase in the growth in vitro of probiotics, Lactobacillus plantarum FMNP01 and Lactobacillus casei FMNP02. After feeding M. basjoo with the middle dose (2.67 g/kg·BW) to mice for 21 days, gut microbiota from mice feces was isolated and sequenced. Results of 16SrDNA sequencing showed that the scattered genera of gut microbiota were significantly gathered. The amounts of different pathogens were decreased, while probiotics such as genera Bacteroides and Roseburia were significantly increased (p < 0.05). Results of function prediction indicated that the reconstruction of gut microbiota may due to the change in carbohydrate transportation, biosynthesis of cell wall, cell membrane, and cell envelope. This study has drawn a basic mechanism in reconstructing gut microbiota by feeding M. basjoo and lay out a foundation for further reach on the interaction between human as diner and M. basjoo as food.
麻竹是中国南方地区一种常见的瘦身水果。然而，尽管其营养成分和生理作用已经得到了很好的研究，但它在重建肠道微生物群方面的内部机制仍不清楚。在这项研究中，将麻竹的成熟度分为四个等级。结果表明，在等级Ⅱ（成熟度为 35%）时，麻竹对益生菌植物乳杆菌 FMNP01 和鼠李糖乳杆菌 FMNP02 的体外生长促进作用最大。用中剂量（2.67 g/kg·BW）喂养小鼠 21 天后，从小鼠粪便中分离并测序肠道微生物群。16SrDNA 测序结果表明，肠道微生物群的分散属显著聚集。不同病原体的数量减少，而益生菌属如拟杆菌属和罗斯伯里氏菌属的数量显著增加（p<0.05）。功能预测结果表明，肠道微生物群的重建可能是由于碳水化合物运输、细胞壁、细胞膜和包膜生物合成的改变。本研究通过喂养麻竹描绘了重建肠道微生物群的基本机制，为进一步研究人类作为食客与麻竹作为食物之间的相互作用奠定了基础。
