Guangdong Provincial Engineering Technology Research Center for Drug and Food Biological Resources Processing and Comprehensive Utilization, School of Life Sciences, South China Normal University, Guangzhou, 510631, China; Guangzhou Key Laboratory of Environmental Catalysis and Pollution Control, School of Environmental Science and Engineering, Institute of Environmental Health and Pollution Control, Guangdong University of Technology, Guangzhou, 51006, China.
Guangdong Provincial Engineering Technology Research Center for Drug and Food Biological Resources Processing and Comprehensive Utilization, School of Life Sciences, South China Normal University, Guangzhou, 510631, China.
Chemosphere. 2019 Jul;226:545-552. doi: 10.1016/j.chemosphere.2019.03.177. Epub 2019 Mar 30.
Bisphenol A (BPA), a plastic additive, is ubiquitous in the environment and has endocrine disrupting effects. As many countries have prohibited the manufacture and sale of plastic products with BPA, BPA analogs have been used to replace BPA during production, including bisphenol S (BPS) and bisphenol B (BPB). To investigate the toxicities of BPA and its analogs on neurons, reactive oxygen species (ROS) assay, Annexin V-FITC (fluorescein) apoptosis detection assay, lactate dehydrogenase (LDH) cytotoxicity assay, and Cell Counting Kit-8 assay were conducted to comprehensively assess the influence of different concentrations of BPA, BPB, and BPS on ROS, apoptosis, damage, and proliferation for hippocampal HT-22 cells, respectively. Results showed that 6 h of exposure to bisphenols (BPs) could increase the ROS levels, 24 h and 48 h of exposure could induce higher apoptosis and LDH leakage rates for HT-22 cells, and 7 d of exposure could inhibit the cell proliferations. In addition, non-monotonic dose-response relationships were observed between the concentrations of bisphenols and the toxic effects mentioned above. The neurotoxic effects of BPA, BPB and BPS on HT-22 cells were in the increasing order of BPS, BPA, and BPB. In conclusion, these results showed that exposure to BPA and its analogs may result in adverse effects on hippocampal neuronal cell lines. BPS is a surrogate with lower neurotoxicity to replace BPA in production of plastic utensils.
双酚 A（BPA）是一种塑料添加剂，在环境中普遍存在，具有内分泌干扰作用。由于许多国家已经禁止制造和销售含有 BPA 的塑料制品，因此在生产过程中已使用 BPA 类似物来替代 BPA，包括双酚 S（BPS）和双酚 B（BPB）。为了研究 BPA 及其类似物对神经元的毒性，进行了活性氧（ROS）测定、Annexin V-FITC（荧光素）凋亡检测、乳酸脱氢酶（LDH）细胞毒性测定和 Cell Counting Kit-8 测定，以综合评估不同浓度的 BPA、BPB 和 BPS 对海马 HT-22 细胞的 ROS、凋亡、损伤和增殖的影响。结果表明，暴露于双酚类化合物（BPs） 6 h 可增加 ROS 水平，暴露 24 h 和 48 h 可诱导 HT-22 细胞更高的凋亡和 LDH 漏出率，暴露 7 d 可抑制细胞增殖。此外，双酚类化合物浓度与上述毒性作用之间存在非单调剂量反应关系。BPA、BPB 和 BPS 对 HT-22 细胞的神经毒性作用按 BPS、BPA 和 BPB 的顺序递增。总之，这些结果表明，暴露于 BPA 及其类似物可能对海马神经元细胞系产生不良影响。BPS 是一种替代物，可在生产塑料器具时替代 BPA，具有较低的神经毒性。
