Hossain Md Shahadat, Hasanuzzaman Mirza, Sohag Md Mahmodul Hasan, Bhuyan M H M Borhannuddin, Fujita Masayuki
1Laboratory of Plant Stress Responses, Faculty of Agriculture, Kagawa University, Miki-cho, Kita-gun, Kagawa 761-0795 Japan.
2Department of Agronomy, Faculty of Agriculture, Sher-e-Bangla Agricultural University, Dhaka, 1207 Bangladesh.
Physiol Mol Biol Plants. 2019 Mar;25(2):443-455. doi: 10.1007/s12298-018-00640-6. Epub 2019 Feb 6.
Physiological and biochemical changes in six-day-old hydroponically grown lentil seedlings exposed to 100 mM salinity stress with or without 5 and 10 mM Na-acetate were studied. Results showed that salt stress reduced recovery percentage, fresh weight (FW), chlorophyll (chl) content, disturbed water balance, disrupted antioxidant defense pathway by decreasing reduced ascorbate content, and caused ion toxicity resulting from increased Na accumulation, severe K loss from roots in hydroponic culture. However, exogenous application of Na-acetate improved the seedling growth by maintaining water balance and increasing chl content. Furthermore, Na-acetate application reduced oxidative damage by modulating antioxidant defense pathway, and sustained ion homeostasis by reducing Na uptake and K loss. In the second experiment in glass house, we investigated the role of Na-acetate on lentil for long-term salinity. Acetate application increased FW and dry weight, reduced oxidative and membrane damage, and lowered the accumulation of Na in shoot compared with salt stressed seedlings alone. From the results of both experiments, it is clear that the exogenous application of Na-acetate enhanced salt tolerance in lentil seedlings.
研究了水培6天的小扁豆幼苗在100 mM盐胁迫下，添加或不添加5 mM和10 mM乙酸钠时的生理生化变化。结果表明，盐胁迫降低了恢复率、鲜重（FW）、叶绿素（chl）含量，扰乱了水分平衡，通过降低还原型抗坏血酸含量破坏了抗氧化防御途径，并导致离子毒性，这是由于水培中钠积累增加、根系严重钾流失所致。然而，外源施加乙酸钠通过维持水分平衡和增加叶绿素含量改善了幼苗生长。此外，施加乙酸钠通过调节抗氧化防御途径减少了氧化损伤，并通过减少钠吸收和钾流失维持了离子稳态。在温室的第二项实验中，我们研究了乙酸钠对小扁豆长期盐胁迫的作用。与单独盐胁迫的幼苗相比，施加乙酸钠增加了鲜重和干重，减少了氧化和膜损伤，并降低了地上部钠的积累。从两项实验的结果来看，很明显外源施加乙酸钠增强了小扁豆幼苗的耐盐性。
