Kaur Ekumjot, Bhardwaj Rachana D, Kaur Simarjit, Grewal Satvir K
Department of Biochemistry, Punjab Agricultural University, Ludhiana, 141004, India.
Biol Futur. 2021 Sep;72(3):347-358. doi: 10.1007/s42977-021-00084-2. Epub 2021 Apr 22.
In the present investigation, influence of water stress on redox metabolism was evaluated in the flag leaf and grains of four barley (Hordeum vulgare L.) genotypes viz DWRB 101, 432 ICARDA, Jyoti and 430 ICARDA at 10th, 20th and 30th days after anthesis (DAA). Relative water content, electrolyte leakage, antioxidative enzymes and their related metabolites were studied during drought stress. Relative water content was well maintained in both the tissues of DWRB 101 and 432 ICARDA. The upregulation of catalase at 20th DAA while ascorbate peroxidase, glutathione reductase and dehydro reductase at 30th DAA in the flag leaf and grains of DWRB 101 and 432 ICARDA may be responsible for lesser increase in H2O2 content as compared to other genotypes. Moreover, the downregulation of superoxide dismutase was comparatively higher in Jyoti and 430 ICARDA. The redox homeostasis was well established during the stress in DWRB 101 and 432 ICARDA by maintaining comparatively higher ratios of ascorbate/dehydroascorbate and reduced/oxidized glutathione. Therefore, scrutiny of data indicated that DWRB 101 and 432 ICARDA may perform better under drought stress in comparison with Jyoti and 430 ICARDA.
在本研究中，于开花后第10天、20天和30天，评估了水分胁迫对四种大麦（Hordeum vulgare L.）基因型即DWRB 101、432 ICARDA、Jyoti和430 ICARDA旗叶和籽粒中氧化还原代谢的影响。在干旱胁迫期间，研究了相对含水量、电解质渗漏、抗氧化酶及其相关代谢产物。DWRB 101和432 ICARDA的两个组织中的相对含水量均保持良好。DWRB 101和432 ICARDA旗叶和籽粒中，过氧化氢酶在开花后第20天上调，而抗坏血酸过氧化物酶、谷胱甘肽还原酶和脱氢还原酶在开花后第30天上调，这可能是与其他基因型相比，其H2O2含量增加较少的原因。此外，Jyoti和430 ICARDA中超氧化物歧化酶的下调相对较高。通过维持相对较高的抗坏血酸/脱氢抗坏血酸和还原型/氧化型谷胱甘肽比率，DWRB 101和432 ICARDA在胁迫期间建立了良好的氧化还原稳态。因此，数据审查表明，与Jyoti和430 ICARDA相比，DWRB 101和432 ICARDA在干旱胁迫下可能表现更好。
