利用基因靶向标记CBDP和SCoT揭示了微繁殖的Lam.中的遗传同质性。
Genetic homogeneity revealed in micropropagated Lam. using gene targeted markers CBDP and SCoT.
作者信息
Sharma Udit, Rai Manoj K, Shekhawat N S, Kataria Vinod
机构信息
1Biotechnology Unit, Department of Botany (UGC-Centre of Advanced Study), Jai Narain Vyas University, New Campus, Jodhpur, Rajasthan 342001 India.
2Department of Environmental Science, Indira Gandhi National Tribal University, Amarkantak, M.P. 484887 India.
出版信息
Physiol Mol Biol Plants. 2019 Mar;25(2):581-588. doi: 10.1007/s12298-018-00639-z. Epub 2019 Feb 6.
Abstract
Two gene targeted markers i.e. CAAT box-derived polymorphism (CBDP) and start codon targeted (SCoT) polymorphism were applied to analyze the genetic stability of in vitro propagated plants of Lam. multiplied by enhanced axillary shoot proliferation of mature tree derived nodal explant. Nine randomly selected micropropagated plants of 1 year age were subjected to molecular analysis. The isolated genomic DNA samples were subjected to PCR amplification with a total of 61 primers (25 CBDP and 36 SCoT) out of which 39 primers (21 CBDP and 18 SCoT) produced scorable amplicons. A total of 97 and 88 clear, distinct and reproducible amplicons were produced by CBDP and SCoT primers, respectively. The monomorphic banding pattern obtained through all the tested primers corroborated the true to type nature of in vitro propagated plants of .
摘要
应用两个基因靶向标记，即CAAT盒衍生多态性（CBDP）和起始密码子靶向（SCoT）多态性，来分析通过成熟树衍生的节段外植体腋芽增殖增强繁殖的罗勒属植物离体繁殖植株的遗传稳定性。选取9株随机挑选的1年生微繁殖植株进行分子分析。将分离的基因组DNA样本用总共61种引物（25种CBDP和36种SCoT）进行PCR扩增，其中39种引物（21种CBDP和18种SCoT）产生了可评分的扩增子。CBDP和SCoT引物分别产生了97条和88条清晰、独特且可重复的扩增子。通过所有测试引物获得的单态条带模式证实了罗勒属植物离体繁殖植株的类型真实性。
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