Department of General Surgery, The First Affiliated Hospital of Shandong First Medical University & Shandong Provincial Qianfoshan Hospital, Jinan 250014, Shandong, China.
Key Laboratory of Metabolism and Gastrointestinal Tumor, the First Affiliated Hospital of Shandong First Medical University, Jinan 250014, Shandong, China.
Cell Cycle. 2021 Apr;20(7):689-701. doi: 10.1080/15384101.2021.1899519. Epub 2021 Mar 22.
It was reported that circular RNA (circRNA) circSMARCA5, as a tumor-related molecule, could modulate development of cancers, including prostatic cancer and cervical cancer. Nevertheless, the essential function of circSMARCA5 in colon cancer has not yet been confirmed. We aimed to investigate the role of circSMARCA5 in colon cancer. CircSMARCA5 expression in tumor cells was detected using RT-qPCR. CCK-8, colony formation, flow cytometry and Transwell assays evaluated the influences of circSMARCA5 in colon cancer cells. RT-qPCR, prediction database and luciferase report assay were accomplished for revealing the correlation between circSMARCA5 and miR-552. After transfection with miR-552 mimic, colon cancer cell behaviors were re-evaluated. Wnt and YAP1 pathways were explored by western blot. Our data presented that circSMARCA5 was under-expressed in colon cancer tissues. Transfection with overexpressing circSMARCA5 plasmid restrained growth, migration and invasion of colon cancer cells. Besides, circSMARCA5 directly sponged to miR-552 and miR-552 up-regulation offset the effects of circSMARCA5 on SW480 and SW620 cells. Furthermore, circSMARCA5 inactivated Wnt and YAP1 pathways by inhibiting miR-552. Anti-tumor role of sircSMARCA5 was showed in colon cancer cells as sponging miR-552 and blocking Wnt and YAP1 pathways.
据报道，环状 RNA（circRNA）circSMARCA5 作为一种与肿瘤相关的分子，可以调节癌症的发展，包括前列腺癌和宫颈癌。然而，circSMARCA5 在结肠癌中的基本功能尚未得到证实。我们旨在研究 circSMARCA5 在结肠癌中的作用。使用 RT-qPCR 检测肿瘤细胞中的 circSMARCA5 表达。CCK-8、集落形成、流式细胞术和 Transwell 分析评估了 circSMARCA5 对结肠癌细胞的影响。通过 RT-qPCR、预测数据库和荧光素酶报告实验完成了 circSMARCA5 与 miR-552 之间的相关性研究。转染 miR-552 模拟物后，重新评估了结肠癌细胞的行为。通过 Western blot 探索了 Wnt 和 YAP1 通路。我们的数据表明，circSMARCA5 在结肠癌组织中表达下调。过表达 circSMARCA5 质粒的转染抑制了结肠癌细胞的生长、迁移和侵袭。此外，circSMARCA5 可以直接与 miR-552 结合，而 miR-552 的上调可以抵消 circSMARCA5 对 SW480 和 SW620 细胞的影响。此外，circSMARCA5 通过抑制 miR-552 使 Wnt 和 YAP1 通路失活。circSMARCA5 通过海绵吸附 miR-552 并阻断 Wnt 和 YAP1 通路，在结肠癌细胞中发挥抗肿瘤作用。
