Moradi Seiran, Anarjan Navideh
Department of Chemical Engineering, Tabriz Branch, Islamic Azad University, Tabriz, Iran.
Food Sci Biotechnol. 2018 Oct 8;28(2):413-421. doi: 10.1007/s10068-018-0478-y. eCollection 2019 Apr.
The preparation of water dispersed α-tocopherol nanocapsules through solvent-displacement technique using gum Arabic (GA) as natural stabilizing and emulsifying biopolymer, for a first time was aimed in current research. The effects of GA concentrations on physicochemical and biological characteristics of prepared nanocapsules, namely, mean particle size, size distribution, zeta potential, rheological properties, turbidity, in vitro antioxidant activity and cellular uptake were evaluated, subsequently. The result indicated that the mono modal size distributed water dispersible α-tocopherol nanocapsules could be successfully attained using selected technique in sizes ranged from 10.01 to 171.2 nm and zeta potential of - 13.5 to - 47.8 mv. The prepared nanocapsules showed the dilatant rheological properties and acceptable radical scavenging (antioxidant activity). The cellular uptake of samples were increased up to 12 times more than microsized α-tocopherol. Consequently, the prepared water dispersed nanosized α-tocopherol can effectively be used in water based food and beverage formulations as nutrition enhancer or natural preservatives.
本研究首次旨在通过溶剂置换技术，以阿拉伯胶（GA）作为天然稳定和乳化生物聚合物来制备水分散性α-生育酚纳米胶囊。随后评估了GA浓度对所制备纳米胶囊的物理化学和生物学特性的影响，即平均粒径、粒径分布、zeta电位、流变学性质、浊度、体外抗氧化活性和细胞摄取。结果表明，使用选定技术能够成功获得单峰粒径分布的水分散性α-生育酚纳米胶囊，其粒径范围为10.01至171.2纳米，zeta电位为-13.5至-47.8毫伏。所制备的纳米胶囊表现出膨胀性流变学性质和可接受的自由基清除能力（抗氧化活性）。样品的细胞摄取比微米级α-生育酚增加了多达12倍。因此，所制备的水分散性纳米级α-生育酚可有效地用于水性食品和饮料配方中，作为营养增强剂或天然防腐剂。
