Department of Veterinary and Animal Sciences, University of Copenhagen, Grønnegårdsvej 15, 1870 Frederiksberg C, Denmark.
Department of Food Science, University of Copenhagen, Rolighedsvej 26, 1958 Frederiksberg C, Denmark.
J Diabetes Res. 2019 Mar 6;2019:1649279. doi: 10.1155/2019/1649279. eCollection 2019.
Lacking the initial contact between the immune system and microbial-associated molecular patterns (MAMPs), such as lipopolysaccharides (LPS), early in life, may be regarded as one of the causal factors of the increasing global increase in the incidence of autoimmune diseases, such as type 1 diabetes (T1D). Previously, a reduced incidence of T1D accompanied by dramatically increased abundances of both the mucin-metabolising bacterium , and LPS-carrying Proteobacteria was observed, when vancomycin was given to pups of nonobese diabetic (NOD) mice. While the T1D incidence reducing effect of . has been shown in further studies, little is known as to whether the increased abundance of LPS-carrying bacteria also has a protective effect. Therefore, we fed NOD pups with LPS orally from birth to weaning, which decreased the gene expressions of , , , , , , , and in the pancreatic lymph nodes, while the same gene expression profile in the spleen was unaffected. However, no significant difference in the incidence of T1D, gut microbiota composition, or ileum expression of the genetic markers of gut permeability, , , -, , , and were observed in relation to LPS ingestion. It is, therefore, concluded that early life oral . LPS has an impact on the local immune response, which, however, did not influence T1D incidence in NOD mice later in life.
在生命早期缺乏免疫系统与微生物相关分子模式（MAMPs）之间的初始接触，例如脂多糖（LPS），可能被认为是全球自身免疫性疾病（如 1 型糖尿病（T1D））发病率不断增加的一个原因。此前，当给予非肥胖型糖尿病（NOD）小鼠的幼崽万古霉素时，观察到 T1D 的发病率降低，同时黏蛋白代谢细菌和携带 LPS 的变形菌的丰度显著增加。虽然已经在进一步的研究中证明了 的 T1D 发病率降低作用，但对于携带 LPS 的细菌丰度增加是否也具有保护作用知之甚少。因此，我们从出生到断奶期间给 NOD 幼崽口服 LPS，这降低了胰腺淋巴结中 、 、 、 、 、 和 的基因表达，而脾脏中的相同基因表达谱不受影响。然而，与 LPS 摄入相关，在 T1D 发病率、肠道微生物群落组成或回肠肠道通透性的遗传标志物 、 、 - 、 、 和 的表达方面，未观察到显著差异。因此，结论是，生命早期口服 LPS 对局部免疫反应有影响，但这并没有影响 NOD 小鼠以后的 T1D 发病率。
