Chen Wenjuan, Zhang Mingming, Shen Wei, Du Bo, Yang Jing, Zhang Qiqing
Tianjin Key Laboratory of Biomedical Materials, Institute of Biomedical Engineering, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300192, China.
Institute of Biomedical and Pharmaceutical Technology, Fuzhou University, Fuzhou 350002, China.
Polymers (Basel). 2019 Jan 3;11(1):60. doi: 10.3390/polym11010060.
The combination of drug and gene strategies for cancer therapy, has exhibited greater effectiveness than drug or gene therapy alone. In this paper, a coil-comb shaped polycationic brush was used as a multifunctional carrier for co-delivery of drug and gene. The side chains of the comb block of the brush were composed of cyclodextrin (CD)-containing cationic star polymers, with a super-high density of positive charge. Doxorubicin (DOX) could be loaded into the cavity of CD polymers to form DOX-loaded nanoparticles (DOX-NPs) and the p53 gene could be subsequently condensed by DOX-NPs. The obtained DOX-NPs/pDNA complexes were less than 150 nm in size, and so could transport DOX and the gene into the same cell. The complexes performed well with regards to their transfection efficiency on MCF-7 cancer cells. As a result, enhanced cell growth inhibition, with decreased DOX dosage was achieved due to the synergistic effect of co-delivery of DOX and the p53 gene. This finding provides an efficient approach for the development of a co-delivery system in combination therapy.
用于癌症治疗的药物和基因策略相结合，已显示出比单独使用药物或基因疗法更高的有效性。在本文中，一种线圈梳状聚阳离子刷被用作药物和基因共递送的多功能载体。该刷梳状嵌段的侧链由含环糊精（CD）的阳离子星形聚合物组成，具有超高密度的正电荷。阿霉素（DOX）可加载到CD聚合物的空腔中形成载DOX纳米颗粒（DOX-NPs），随后p53基因可被DOX-NPs浓缩。所获得的DOX-NPs/pDNA复合物尺寸小于150nm，因此能够将DOX和基因转运到同一个细胞中。这些复合物在MCF-7癌细胞上的转染效率良好。结果，由于DOX和p53基因共递送的协同作用，实现了增强的细胞生长抑制，同时降低了DOX剂量。这一发现为联合治疗中共递送系统的开发提供了一种有效方法。
