Department of gastroenterology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, Hubei Province, China.
Department of Emergency Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, Hubei Province, China
Biosci Rep. 2019 May 17;39(5). doi: 10.1042/BSR20182498. Print 2019 May 31.
Previous study has explored that SNHG16, a long non-coding RNA (lncRNA), mediated cell growth and proliferation. Yet, the role of SNHG16 in human colorectal cancer (CRC) still remains to be explored. Therefore, we conducted the present study to explore the functions of SNHG16 in CRC. In the present study, SNHG16 was significantly up-regulated in CRC tissues and cell lines. Gain- and loss-of-function of SNHG16 further presented that SNHG16 promoted the progression of CRC cells, including proliferation, migration, and invasion. Further, study also revealed that overexpression of SNHG16 could promote tumor growth. Bioinformatics analysis and luciferase reporter assay showed that SNHG16 was a direct target of miR-200a-3p. MiR-200a-3p was inversely correlated with SNHG16 expression in CRC tissues. In brief, the above results elucidate the important role of SNHG16 in CRC tumorigenesis, suggesting that SNHG16 might be quite vital for the diagnosis and development of CRC.
先前的研究已经探讨了 SNHG16，一种长链非编码 RNA（lncRNA），可介导细胞生长和增殖。然而，SNHG16 在人结直肠癌（CRC）中的作用仍有待探索。因此，我们进行了本研究以探讨 SNHG16 在 CRC 中的功能。在本研究中，SNHG16 在 CRC 组织和细胞系中显著上调。SNHG16 的功能获得和缺失实验进一步表明，SNHG16 促进了 CRC 细胞的进展，包括增殖、迁移和侵袭。此外，研究还揭示了过表达 SNHG16 可以促进肿瘤生长。生物信息学分析和荧光素酶报告基因实验表明，SNHG16 是 miR-200a-3p 的直接靶标。miR-200a-3p 在 CRC 组织中与 SNHG16 的表达呈负相关。总之，上述结果阐明了 SNHG16 在 CRC 肿瘤发生中的重要作用，表明 SNHG16 可能对 CRC 的诊断和发展至关重要。
