Tan Pei, Xu Mu, Nie Junjie, Qin Jian, Liu Xiangxiang, Sun Huiling, Wang Shukui, Pan Yuqin
General Clinical Research Center, Nanjing First Hospital, Nanjing Medical University, Nanjing, Jiangsu 210006, China.
Department of Laboratory Medicine, Nanjing First Hospital, Nanjing Medical University, Nanjing, Jiangsu 210006, China.
J Biomed Res. 2022 Jun 28;36(4):231-241. doi: 10.7555/JBR.36.20220049.
Mounting evidence indicates that long non-coding RNAs (lncRNAs) have critical roles in colorectal cancer (CRC) progression, providing many potential diagnostic biomarkers, prognostic biomarkers, and treatment targets. Here, we sought to investigate the role and underlying regulatory mechanism of lncRNA small nucleolar RNA host gene 16 ( ) in CRC. The expressions of in CRC were identified by RNA-sequencing and quantitative reverse transcription PCR. The functions of were explored by a series of and assays (colony formation assay, flow cytometry assay, and xenograft model). Bioinformatics analysis, RNA fluorescence hybridization and luciferase reporter assay were used to investigate the regulatory mechanism of effects of . was found to be significantly elevated in human CRC tissues and cell lines. Functional studies suggested that promoted CRC cell growth both and . Mechanistically, we identified that is expressed predominantly in the cytoplasm. could interact with miR-214-3p and up-regulated its target ABCB1. This study indicated that plays an oncogenic role in CRC, suggesting it could be a novel biomarker and therapeutic target in CRC.
越来越多的证据表明，长链非编码RNA（lncRNAs）在结直肠癌（CRC）进展中起关键作用，提供了许多潜在的诊断生物标志物、预后生物标志物和治疗靶点。在此，我们试图研究lncRNA小核仁RNA宿主基因16（ ）在CRC中的作用及其潜在调控机制。通过RNA测序和定量逆转录PCR鉴定CRC中 的表达。通过一系列 和 实验（集落形成实验、流式细胞术实验和异种移植模型）探索 的功能。采用生物信息学分析、RNA荧光 杂交和荧光素酶报告基因实验来研究 作用的调控机制。发现 在人CRC组织和细胞系中显著升高。功能研究表明， 在 和 方面均促进CRC细胞生长。从机制上讲，我们确定 主要在细胞质中表达。 可与miR-214-3p相互作用并上调其靶标ABCB1。本研究表明， 在CRC中起致癌作用，提示它可能是CRC中的一种新型生物标志物和治疗靶点。
