ICAR-National Rice Research Institute, Cuttack, Odisha, 753006, India.
Institute of Life Sciences, Nalco Square, Bhubaneswar, Odisha, 751023, India.
Sci Rep. 2019 Apr 8;9(1):5753. doi: 10.1038/s41598-019-42170-5.
Low light intensity is a great limitation for grain yield and quality in rice. However, yield is not significantly reduced in low light tolerant rice varieties. The work therefore planned for comparative transcriptome profiling under low light stress to decipher the genes involved and molecular mechanism of low light tolerance in rice. At active tillering stage, 50% low light exposure for 1 day, 3 days and 5 days were given to Swarnaprabha (low light tolerant) and IR8 (low light sensitive) rice varieties. Illumina (HiSeq) platform was used for transcriptome sequencing. A total of 6,652 and 12,042 genes were differentially expressed due to low light intensity in Swarnaprabha and IR8, respectively as compared to control. CAB, LRP, SBPase, MT15, TF PCL1 and Photosystem I & II complex related gene expressions were mostly increased in Swarnaprabha upon longer duration of low light exposure which was not found in IR8 as compared to control. Their expressions were validated by qRT-PCR. Overall study suggested that the maintenance of grain yield in the tolerant variety under low light might be results of accelerated expression of the genes which enable the plant to keep the photosynthetic processes moving at the same pace even under low light.
弱光强度是限制水稻籽粒产量和品质的主要因素。然而，在耐低光品种中，产量并没有显著降低。因此，本研究计划进行比较转录组分析，以揭示水稻耐低光的相关基因和分子机制。在活跃分蘖期，将 Swarnaprabha（耐低光）和 IR8（低光敏感）水稻品种暴露于 50%低光强度下 1 天、3 天和 5 天。使用 Illumina（HiSeq）平台进行转录组测序。与对照相比，Swarnaprabha 和 IR8 分别有 6652 个和 12042 个基因因低光强度而差异表达。与对照相比，在较长时间的低光暴露下，CAB、LRP、SBPase、MT15、TF PCL1 和光系统 I 和 II 复合物相关基因在 Swarnaprabha 中的表达大多增加，而在 IR8 中则没有发现这种情况。通过 qRT-PCR 验证了它们的表达。总的来说，该研究表明，在耐低光品种中，维持籽粒产量可能是由于这些基因的表达加速，使植物即使在低光下也能保持光合作用的进程。
