Arguedas M, Villalobos A, Gómez D, Hernández L, Zevallos B E, Cejas I, Yabor L, Martínez-Montero M Edel, Lorenzo J Carlos
Laboratory for Plant Breeding and Conservation of Genetic Resources, Centro de Bioplantas, Universidad de Ciego de Ávila, Ciego de Ávila 69450, Cuba.
Escuela Superior Politécnica Agropecuaria de Manabí Manuel Félix López (ESPAMMFL), Campus Politécnico El Limón, Carrera de Ingeniería Agrícola, Calceta, Manabí, Ecuador.
Cryo Letters. 2018 Nov/Dec;39(6):366-370.
Cryo-preservation of plant materials in liquid nitrogen (LN) has been described as a suitable technology to conserve genetic resources of several species. However, the potential effects of LN in the subsequent plant growth in the field should be studied before large-scale implementation of cryopreserved germplasm banks.
To describe the field performance of cryopreserved seed-derived maize adult plants.
Germination percentage and numbers of leaves and ears per plant, internodes in stems, middle - aged leaf length, plant height, ear traits and weight of 100 seeds were recorded.
Statistically significant differences between adult plants derived from cryopreserved seeds and the control treatment were not observed (t-test, p=0.05).
The results presented confirm at the phenotype level the effectiveness of maize seed cryostorage to preserve and regenerate true-to-type plants.
液氮（LN）中植物材料的冷冻保存已被描述为一种保存多个物种遗传资源的合适技术。然而，在大规模实施冷冻保存种质库之前，应研究液氮对后续田间植物生长的潜在影响。
描述冷冻保存的种子衍生玉米成年植株的田间表现。
记录发芽率、每株植物的叶片和果穗数量、茎节数、中叶长度、株高、果穗性状以及100粒种子的重量。
未观察到冷冻保存种子衍生的成年植株与对照处理之间存在统计学上的显著差异（t检验，p = 0.05）。
所呈现的结果在表型水平上证实了玉米种子冷冻保存用于保存和再生典型植株的有效性。
