Jehan Mehreen, Saeed Fiza, Khan Zanib, Shah Muddassar, Sikandar Ayesha, Inayat Afia, Ali Sartaj, Mehmood Khan Arshad, Talib Ayesha, Aasim Muhammad, Ali Khan Abid
Department of Microbiology, Government Postgraduate College No. 2, Mandian, Abbottabad, KP, Pakistan.
Department of Biosciences, COMSATS University Islamabad, Tarlai Kalan, 45550 Islamabad, Pakistan.
IET Nanobiotechnol. 2018 Dec;12(8):1062-1066. doi: 10.1049/iet-nbt.2018.5054.
is used for medicinal purposes for a long time all over the world. In this study, the authors report the antimicrobial potential of extracts obtained with different solvents. The extract(s) obtained with ethyl acetate yielded the best antibacterial effects because of a rich supply of oxalates and alkaloids in it. The same extract was also exploited for reducing Ag ions (to metallic Ag) for the synthesis of nanoparticles. Electron microscopy revealed that the silver nanoparticles were ∼18-25 nm in diameter. The Fourier-transform infrared evaluation pointed towards the fact that flavonoids present in the plant extract were acting as reductants while amino groups were the bound stabilisation agents to the synthesised nanoparticles limiting the diameter to a certain threshold and avoiding aggregation naturally. A comparative antibacterial assay of versus Ag nanoparticles showed that the nanoparticles as well as organic (ethyl acetate) extract of the plant checked the growth of selected (MDR) superbugs. However, the biosynthesised Ag nanoparticles returned better antibacterial efficacies than ethyl acetate extract.
在世界各地，它长期被用于药用目的。在本研究中，作者报告了用不同溶剂获得的提取物的抗菌潜力。用乙酸乙酯获得的提取物产生了最佳抗菌效果，因为其中富含草酸盐和生物碱。同样的提取物还被用于还原银离子（生成金属银）以合成纳米颗粒。电子显微镜显示银纳米颗粒的直径约为18 - 25纳米。傅里叶变换红外光谱评估表明，植物提取物中存在的黄酮类化合物充当还原剂，而氨基是合成纳米颗粒的结合稳定剂，将直径限制在一定阈值并自然避免聚集。对植物提取物与银纳米颗粒的比较抗菌试验表明，纳米颗粒以及植物的有机（乙酸乙酯）提取物抑制了所选多重耐药超级细菌的生长。然而，生物合成的银纳米颗粒比乙酸乙酯提取物具有更好的抗菌效果。
