Chongming Branch Urology Surgery, Xinhua Hospital Affiliated to Shanghai Jiaotong University School of Medicine, Shanghai, China.
Eur Rev Med Pharmacol Sci. 2020 Jan;24(2):633-638. doi: 10.26355/eurrev_202001_20039.
Prostate cancer is one of the most ordinary malignant tumors. Recently, the role of long non-coding RNAs (lncRNAs) in tumor progression has caught the attention of numerous researchers. In this work, lncRNA SNHG14 was studied to identify how it functioned in the progression of prostate cancer.
First, Real Time-quantitative Polymerase Chain Reaction (RT-qPCR) was utilized to measure SNHG14 expression in prostate cancer tissues and cell lines. Furthermore, to identify the function of SNHG14 in prostate cancer, functional experiments were conducted in vitro and in vivo. In addition, by performing Luciferase assays and RNA immunoprecipitation assay (RIP), the underlying mechanism was explored.
In this work, SNHG14 expression was remarkably higher in prostate cancer samples when compared with that in the corresponding ones. Moreover, cell proliferation was inhibited after SNHG14 was silenced in prostate cancer cells and the expression of miR-613 was upregulated after SNHG14 was silenced. Further mechanism assays showed that miR-613 was a direct target of SNHG14 in prostate cancer. In addition, tumor formation was inhibited after SNHG14 was knocked-down in vivo.
Our study discovers a potential oncogene in prostate cancer and identifies that SNHG14 enhances cell proliferation via sponging miR-613.
前列腺癌是最常见的恶性肿瘤之一。最近，长链非编码 RNA（lncRNA）在肿瘤进展中的作用引起了众多研究人员的关注。在这项工作中，研究了 lncRNA SNHG14，以确定其在前列腺癌进展中的作用方式。
首先，利用实时定量聚合酶链反应（RT-qPCR）测量前列腺癌组织和细胞系中的 SNHG14 表达。此外，为了确定 SNHG14 在前列腺癌中的功能，在体外和体内进行了功能实验。此外，通过进行荧光素酶测定和 RNA 免疫沉淀测定（RIP），探讨了潜在的机制。
在这项工作中，与相应的组织相比，前列腺癌样本中的 SNHG14 表达明显升高。此外，沉默前列腺癌细胞中的 SNHG14 后，细胞增殖受到抑制，并且沉默 SNHG14 后 miR-613 的表达上调。进一步的机制分析表明，miR-613 是前列腺癌中 SNHG14 的直接靶标。此外，在体内敲低 SNHG14 后抑制了肿瘤形成。
我们的研究发现了前列腺癌中的一种潜在致癌基因，并确定 SNHG14 通过海绵吸附 miR-613 增强细胞增殖。
