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LncRNA DARS-AS1的下调通过抑制IGF2BP3抑制宫颈癌的肿瘤发生。

Downregulation of LncRNA DARS-AS1 Inhibits the Tumorigenesis of Cervical Cancer via Inhibition of IGF2BP3.

作者信息

Zhu Jinming, Han Shichao

机构信息

Department of Oncology, Affiliated Zhongshan Hospital, Dalian University, Dalian, Liaoning, 116000, People's Republic of China.

Department of Gynecology, The 2nd Affiliated Hospital of Dalian Medical University, Dalian, Liaoning, 116021, People's Republic of China.

出版信息

Onco Targets Ther. 2021 Feb 25;14:1331-1340. doi: 10.2147/OTT.S274623. eCollection 2021.

DOI:10.2147/OTT.S274623
PMID:33658798
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7920590/
Abstract

BACKGROUND

Evidence has been shown that long noncoding RNAs (lncRNAs) play an important role in the development of cervical cancer. Recently, lncRNA DARS-AS1 was reported to be dysregulated in several cancer types; however, the role of DARS-AS1 in cervical cancer remains unclear.

METHODS

Flow cytometry and transwell invasion assays were performed to determine the apoptosis and invasion in cervical cancer cells. In addition, RNA pull-down and fluorescence in situ hybridization (FISH) assays were conducted to assess the interaction between DARS-AS1 and IGF2BP3 in cervical cancer cells.

RESULTS

Downregulation of DARS-AS1 significantly induced apoptosis and cell cycle arrest in cervical cancer cells. Meanwhile, the invasion ability of cervical cancer cells was inhibited by DARS-AS1 knockdown as well. RNA pull-down and FISH results showed that DARS-AS1 interacted with IGF2BP3. Mechanistically, DARS-AS1 positively regulated IGF2BP3 expression via stabilization of IGF2BP3 mRNA. Rescue assays confirmed that DARS-AS1 regulated the progression of cervical cancer through interacting with IGF2BP3 in vitro. In addition, in vivo experiments revealed that downregulation of DARS-AS1 inhibited tumor growth in SiHa xenograft model.

CONCLUSION

In this study, we found that downregulation of DARS-AS1 could inhibit the growth of cervical cancer cells via inhibition of IGF2BP3, suggesting DARS-AS1 might serve as a potential target for the treatment of cervical cancer.

摘要

背景

已有证据表明长链非编码RNA（lncRNA）在宫颈癌的发生发展中起重要作用。最近，有报道称lncRNA DARS-AS1在几种癌症类型中表达失调；然而，DARS-AS1在宫颈癌中的作用仍不清楚。

方法

采用流式细胞术和Transwell侵袭实验来检测宫颈癌细胞的凋亡和侵袭情况。此外，进行RNA下拉实验和荧光原位杂交（FISH）实验，以评估DARS-AS1与宫颈癌细胞中IGF2BP3之间的相互作用。

结果

DARS-AS1的下调显著诱导宫颈癌细胞凋亡和细胞周期停滞。同时，DARS-AS1敲低也抑制了宫颈癌细胞的侵袭能力。RNA下拉实验和FISH结果显示DARS-AS1与IGF2BP3相互作用。机制上，DARS-AS1通过稳定IGF2BP3 mRNA正向调节IGF2BP3的表达。挽救实验证实，DARS-AS1在体外通过与IGF2BP3相互作用调节宫颈癌的进展。此外，体内实验表明，在SiHa异种移植模型中，DARS-AS1的下调抑制了肿瘤生长。

结论

在本研究中，我们发现下调DARS-AS1可通过抑制IGF2BP3来抑制宫颈癌细胞的生长，提示DARS-AS1可能成为治疗宫颈癌的潜在靶点。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4399/7920590/b048f99b60b4/OTT-14-1331-g0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4399/7920590/096fcaaae13a/OTT-14-1331-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4399/7920590/aa6a26909bd9/OTT-14-1331-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4399/7920590/9f11d2723a65/OTT-14-1331-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4399/7920590/0da82556c3d6/OTT-14-1331-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4399/7920590/f4ed0c862b63/OTT-14-1331-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4399/7920590/b048f99b60b4/OTT-14-1331-g0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4399/7920590/096fcaaae13a/OTT-14-1331-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4399/7920590/aa6a26909bd9/OTT-14-1331-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4399/7920590/9f11d2723a65/OTT-14-1331-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4399/7920590/0da82556c3d6/OTT-14-1331-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4399/7920590/f4ed0c862b63/OTT-14-1331-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4399/7920590/b048f99b60b4/OTT-14-1331-g0006.jpg

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2
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3
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Int J Mol Sci. 2023 May 29;24(11):9423. doi: 10.3390/ijms24119423.
4
LINC01082 Inhibits Non-Small Cell Lung Cancer by Targeting the miR-543/TNRC6A Axis.LINC01082 通过靶向 miR-543/TNRC6A 轴抑制非小细胞肺癌。
Biochem Genet. 2023 Aug;61(4):1585-1605. doi: 10.1007/s10528-022-10313-5. Epub 2023 Jan 31.
5
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Stem Cells Int. 2022 Sep 22;2022:4041550. doi: 10.1155/2022/4041550. eCollection 2022.
6
Analysis of lncRNA sequences: FAM3D-AS1, LINC01230, LINC01315 and LINC01468 in endometrial cancer.分析长链非编码 RNA 序列：FAM3D-AS1、LINC01230、LINC01315 和 LINC01468 在子宫内膜癌中的作用。
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7
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Cancer Med. 2020 Jan;9(1):278-289. doi: 10.1002/cam4.2675. Epub 2019 Nov 8.
6
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Exp Mol Pathol. 2019 Dec;111:104322. doi: 10.1016/j.yexmp.2019.104322. Epub 2019 Oct 23.
7
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Haematologica. 2020 Jun;105(6):1630-1640. doi: 10.3324/haematol.2019.218289. Epub 2019 Jul 9.
8
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Cells. 2019 Jun 21;8(6):622. doi: 10.3390/cells8060622.
9
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Papillomavirus Res. 2019 Dec;8:100170. doi: 10.1016/j.pvr.2019.100170. Epub 2019 Jun 6.
10
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