The Key Laboratory of Resource Chemistry of the Ministry of Education, the Shanghai Key Laboratory of Rare Earth Functional Materials, and the Shanghai Municipal Education Committee Key Laboratory of Molecular Imaging Probes and Sensors , Shanghai Normal University , Shanghai 200234 , China.
College of Health Science and Environmental Engineering , Shenzhen Technology University , Shenzhen 518118 , China.
ACS Appl Mater Interfaces. 2019 May 1;11(17):15251-15261. doi: 10.1021/acsami.9b00495. Epub 2019 Apr 17.
Macrophage-mediated delivery of drugs or nanoparticles has great potential in cancer treatment because it can avoid interception by the immune system and cross the blood-vessel barriers to reach the hypoxic regions of tumors. However, macrophage-based delivery system still faces some great challenges such as low theranostics agent loading capacity and hypoxic regions tendency in vivo. Herein, small gold nanorods (AuNRs) were used as the model theranostics agent to design a macrophage-mediated delivery system with high loading quantity for tumor hypoxia photoacoustic (PA) imaging and enhanced photothermal therapy (PTT). AuNRs modified with various thiolated poly(ethylene glycol)s (HS-PEG) via ligand exchange were investigated for toxicity and cell uptake by macrophages. The tumor hypoxic regions tendency of macrophage-loaded Anionic-AuNRs (Anionic-AuNRs@RAW) were verified by in vivo PA imaging and tumor sections. In vivo systemic PTT demonstrated enhanced tumor inhibition of anionic-AuNRs@RAW. This macrophage-mediated delivery system with high loading capacity could be used to enhance the effectiveness of cancer treatment.
巨噬细胞介导的药物或纳米颗粒传递在癌症治疗中有很大的潜力，因为它可以避免被免疫系统拦截，并穿过血管屏障到达肿瘤的缺氧区域。然而，基于巨噬细胞的递药系统仍然面临一些重大挑战，如治疗剂载药量低和体内缺氧区域倾向。在此，小金纳米棒（AuNRs）被用作模型治疗剂，设计了一种具有高载药量的巨噬细胞介导的递药系统，用于肿瘤缺氧光声（PA）成像和增强光热治疗（PTT）。通过配体交换，用各种巯基化聚乙二醇（HS-PEG）修饰 AuNRs，研究其对巨噬细胞的毒性和细胞摄取。通过体内 PA 成像和肿瘤切片验证了载巨噬细胞的阴离子-AuNRs（Anionic-AuNRs@RAW）的肿瘤缺氧区域倾向。体内系统 PTT 显示出增强的阴离子-AuNRs@RAW 肿瘤抑制作用。这种具有高载药量的巨噬细胞介导的递药系统可用于提高癌症治疗的效果。
