Myhrstad Mari C W, de Mello Vanessa D, Dahlman Ingrid, Kolehmainen Marjukka, Paananen Jussi, Rundblad Amanda, Carlberg Carsten, Olstad Ole Kristoffer, Pihlajamäki Jussi, Holven Kirsten B, Hermansen Kjeld, Dragsted Lars O, Gunnarsdottir Ingibjörg, Cloetens Lieselotte, Storm Matilda Ulmius, Åkesson Björn, Rosqvist Fredrik, Hukkanen Janne, Herzig Karl-Heinz, Risérus Ulf, Thorsdottir Inga, Poutanen Kaisa S, Savolainen Markku J, Schwab Ursula, Arner Peter, Uusitupa Matti, Ulven Stine M
Department of Nursing and Health Promotion, Faculty of Health Sciences, Oslo Metropolitan University, 0130, Oslo, Norway.
Institute of Public Health and Clinical Nutrition, University of Eastern Finland, 70211, Kuopio, Finland.
Mol Nutr Food Res. 2019 Jul;63(13):e1801405. doi: 10.1002/mnfr.201801405. Epub 2019 Apr 18.
To explore the effect of a healthy Nordic diet on the global transcriptome profile in peripheral blood mononuclear cells (PBMCs) of subjects with metabolic syndrome.
Subjects with metabolic syndrome undergo a 18/24 week randomized intervention study comparing an isocaloric healthy Nordic diet with an average habitual Nordic diet served as control (SYSDIET study). Altogether, 68 participants are included. PBMCs are obtained before and after intervention and total RNA is subjected to global transcriptome analysis. 1302 probe sets are differentially expressed between the diet groups (p-value < 0.05). Twenty-five of these are significantly regulated (FDR q-value < 0.25) and are mainly involved in mitochondrial function, cell growth, and cell adhesion. The list of 1302 regulated probe sets is subjected to functional analyses. Pathways and processes involved in the mitochondrial electron transport chain, immune response, and cell cycle are downregulated in the healthy Nordic diet group. In addition, gene transcripts with common motifs for 42 transcription factors, including NFR1, NFR2, and NF-κB, are downregulated in the healthy Nordic diet group.
These results suggest that benefits of a healthy diet may be mediated by improved mitochondrial function and reduced inflammation.
探讨健康的北欧饮食对代谢综合征患者外周血单核细胞（PBMC）全球转录组图谱的影响。
代谢综合征患者进行了一项为期18/24周的随机干预研究，将等热量的健康北欧饮食与作为对照的普通北欧习惯饮食进行比较（SYSDIET研究）。总共纳入了68名参与者。在干预前后获取PBMC，并对总RNA进行全球转录组分析。饮食组之间有1302个探针集差异表达（p值<0.05）。其中25个受到显著调控（FDR q值<0.25），主要涉及线粒体功能、细胞生长和细胞粘附。对1302个受调控探针集的列表进行功能分析。健康北欧饮食组中线粒体电子传递链、免疫反应和细胞周期所涉及的途径和过程被下调。此外，包括NFR1、NFR2和NF-κB在内的42种转录因子具有共同基序的基因转录本在健康北欧饮食组中也被下调。
这些结果表明，健康饮食的益处可能通过改善线粒体功能和减轻炎症来介导。
