健康的北欧饮食可下调代谢综合征患者皮下脂肪组织中参与炎症反应的基因表达。
Healthy Nordic diet downregulates the expression of genes involved in inflammation in subcutaneous adipose tissue in individuals with features of the metabolic syndrome.
机构信息
From the Institute of Public Health and Clinical Nutrition (MK, J Paananen, VdM, US, J Pihlajamäki, KSP, and MU) and the Institute of Biomedicine (CC), University of Eastern Finland, Kuopio, Finland; the Institute of Clinical Medicine, Clinical Nutrition (US and J Pihlajamäki) and Research Unit (MU), Kuopio University Hospital, Kuopio, Finland; the Department of Health, Nutrition and Management, Faculty of Health Sciences, Oslo, Norway, and Akershus University College of Applied Sciences, Oslo, Norway (SMU and MM); the Department of Medicine (H7), Karolinska Institute, Stockholm, Sweden (ID, PA, ED, and ES); the Unit for Nutrition Research, University of Iceland and Landspitali-The National University Hospital of Iceland, Reykjavik, Iceland (IG and IT); Biomedical Nutrition, Pure and Applied Biochemistry, Lund University, Lund, Sweden (LC and BÅ); the Departments of Endocrinology (ML-O) and Clinical Nutrition (BÅ), Skåne University Hospital, Lund, Sweden; the Department of Public Health and Caring Sciences, Clinical Nutrition and Metabolism, Uppsala University, Uppsala, Sweden (FR and UR); the Institute of Clinical Medicine, Department of Internal Medicine and Biocenter Oulu, University of Oulu, and Medical Research Center, Oulu University Hospital, Oulu, Finland (MJS and JH); University of Copenhagen, Faculty of Science, Department of Human Nutrition, Copenhagen, Denmark (LOD); Institute of Biomedicine, University of Oulu, Oulu, Finland (K-HH); the Department of Endocrinology and Internal Medicine, Aarhus University Hospital, Aarhus, Denmark (KH and LB); and VTT Technical Research Centre of Finland, Espoo, Finland (KSP).
出版信息
Am J Clin Nutr. 2015 Jan;101(1):228-39. doi: 10.3945/ajcn.114.092783. Epub 2014 Nov 19.
BACKGROUND
Previously, a healthy Nordic diet (ND) has been shown to have beneficial health effects close to those of Mediterranean diets.
OBJECTIVE
The objective was to explore whether the ND has an impact on gene expression in abdominal subcutaneous adipose tissue (SAT) and whether changes in gene expression are associated with clinical and biochemical effects.
DESIGN
Obese adults with features of the metabolic syndrome underwent an 18- to 24-wk randomized intervention study comparing the ND with the control diet (CD) (the SYSDIET study, carried out within Nordic Centre of Excellence of the Systems Biology in Controlled Dietary Interventions and Cohort Studies). The present study included participants from 3 Nordic SYSDIET centers [Kuopio (n = 20), Lund (n = 18), and Oulu (n = 18)] with a maximum weight change of ±4 kg, highly sensitive C-reactive protein concentration <10 mg/L at the beginning and the end of the intervention, and baseline body mass index (in kg/m²) <38. SAT biopsy specimens were obtained before and after the intervention and subjected to global transcriptome analysis with Gene 1.1 ST Arrays (Affymetrix).
RESULTS
Altogether, 128 genes were differentially expressed in SAT between the ND and CD (nominal P < 0.01; false discovery rate, 25%). These genes were overrepresented in pathways related to immune response (adjusted P = 0.0076), resulting mainly from slightly decreased expression in the ND and increased expression in the CD. Immune-related pathways included leukocyte trafficking and macrophage recruitment (e.g., interferon regulatory factor 1, CD97), adaptive immune response (interleukin32, interleukin 6 receptor), and reactive oxygen species (neutrophil cytosolic factor 1). Interestingly, the regulatory region of the 128 genes was overrepresented for binding sites for the nuclear transcription factor κB.
CONCLUSION
A healthy Nordic diet reduces inflammatory gene expression in SAT compared with a control diet independently of body weight change in individuals with features of the metabolic syndrome.
背景
先前的研究表明，健康的北欧饮食（ND）对健康的有益影响接近于地中海饮食。
目的
本研究旨在探索 ND 是否会对腹部皮下脂肪组织（SAT）中的基因表达产生影响，以及基因表达的变化是否与临床和生化效应相关。
设计
患有代谢综合征特征的肥胖成年人参与了一项为期 18-24 周的随机干预研究，比较 ND 与对照饮食（CD）的效果（SYSDIET 研究，在北欧系统生物学卓越中心内进行，该中心进行了受控饮食干预和队列研究中的系统生物学）。本研究纳入了来自 3 个北欧 SYSDIET 中心（于韦斯屈莱[Kuopio]、隆德[Lund]和奥卢[Oulu]）的参与者，他们的体重变化最大为±4kg，干预前后高敏 C 反应蛋白浓度均<10mg/L，且基线体重指数（BMI）<38kg/m²。在干预前后采集 SAT 活检标本，并使用 Gene 1.1 ST Arrays（Affymetrix）进行全转录组分析。
结果
ND 和 CD 之间的 SAT 中有 128 个基因的表达存在差异（名义 P<0.01；错误发现率为 25%）。这些基因在与免疫反应相关的途径中过度表达（调整后的 P=0.0076），主要是由于 ND 组中基因表达略有下降，而 CD 组中基因表达略有增加。与免疫相关的途径包括白细胞迁移和巨噬细胞募集（如干扰素调节因子 1、CD97）、适应性免疫反应（白细胞介素 32、白细胞介素 6 受体）和活性氧（中性粒细胞胞浆因子 1）。有趣的是，128 个基因的调控区存在核转录因子κB 结合位点的过度表达。
结论
与对照饮食相比，健康的北欧饮食可降低代谢综合征特征个体 SAT 中的炎症基因表达，而与体重变化无关。
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