Yasar Hanzey, Ho Duy-Khiet, De Rossi Chiara, Herrmann Jennifer, Gordon Sarah, Loretz Brigitta, Lehr Claus-Michael
Helmholtz Institute for Pharmaceutical Research Saarland (HIPS), Helmholtz Center for Infection Research (HZI), Saarland University, D-66123 Saarbrücken, Germany.
Department of Pharmacy, Saarland University, D-66123 Saarbrücken, Germany.
Polymers (Basel). 2018 Mar 1;10(3):252. doi: 10.3390/polym10030252.
Despite the enormous potential of nanomedicine, the search for materials from renewable resources that balance bio-medical requirements and engineering aspects is still challenging. This study proposes an easy method to make nanoparticles composed of oxidized starch and chitosan, both isolated from natural biopolymers. The careful adjustment of C/N ratio, polymer concentration and molecular weight allowed for tuning of particle characteristics. The system's carrier capability was assessed both for anti-infectives and for nucleic acid. Higher starch content polyplexes were found to be suitable for high encapsulation efficiency of cationic anti-infectives and preserving their bactericidal function. A cationic carrier was obtained by coating the anionic polyplex with chitosan. Coating allowed for a minimal amount of cationic polymer to be employed and facilitated plasmid DNA loading both within the particle core and on the surface. Transfection studies showed encouraging result, approximately 5% of A549 cells with reporter gene expression. In summary, starch-chitosan complexes are suitable carriers with promising perspectives for pharmaceutical use.
尽管纳米医学具有巨大潜力，但从可再生资源中寻找能平衡生物医学需求和工程方面的材料仍具有挑战性。本研究提出了一种简便方法来制备由氧化淀粉和壳聚糖组成的纳米颗粒，二者均从天然生物聚合物中分离得到。通过仔细调整碳/氮比、聚合物浓度和分子量，可以调节颗粒特性。该系统作为抗感染剂和核酸载体的能力均得到了评估。发现较高淀粉含量的多聚体适合高封装阳离子抗感染剂并保留其杀菌功能。通过用壳聚糖包被阴离子多聚体获得了阳离子载体。包被使得阳离子聚合物的用量最少，并促进质粒DNA在颗粒核心内和表面的负载。转染研究显示了令人鼓舞的结果，约5%的A549细胞有报告基因表达。总之，淀粉-壳聚糖复合物是具有良好药用前景的合适载体。
