Department of Dermatology, Heilongjiang Provincial Hospital, Harbin, 150000, China.
Department of Dermatology, Heilongjiang Provincial Hospital, Harbin, 150000, China.
Biochem Biophys Res Commun. 2019 May 28;513(2):380-385. doi: 10.1016/j.bbrc.2019.04.017. Epub 2019 Apr 6.
Accumulating studies have appreciated circular RNAs (circRNAs) as novel prognostic biomarkers and therapeutic targets in malignant carcinomas. Here, we aim to investigate the expression of a novel circRNA, circ_0070934. The biological roles and mechanisms of circ_0070934 in cutaneous squamous cell carcinoma (CSCC) were explored. The expression of circ_0070934 in CSCC tissues and cell lines was evaluated by qRT-PCR. Loss-of-function and gain-of-function assays were performed to detect cell proliferation, apoptosis, migration and invasion in vitro. Moreover, the underlying molecular mechanism of circ_0070934 was predicted by online database and luciferase reporter assay. Abnormally overexpression of circ_0070934 was detected in CSCC samples and cell lines. Inhibition of cell proliferation, invasion, migration, and increased apoptosis were observed upon circ_0070934 knockdown. The opposite effect was observed in the circ_0070934 overexpression cells. Circ_0070934 expression was negatively correlated with miR-1238 and miR-1247-5p expression in CSCC and luciferase reporter experiment verified the binding ability between circ_0070934 and miR-1238/miR-1247-5p. Rescue experiments further identified that the oncogenic role of circ_0070934 is attributed to its suppression of miR-1238 and miR-1247-5p. Taken together, our results implicate that circ_0070934 is correlated with tumor aggressiveness by serving as an oncogenic circRNA in CSCC.
越来越多的研究表明，环状 RNA（circRNAs）作为恶性肿瘤的新型预后生物标志物和治疗靶点。在这里，我们旨在研究一种新型环状 RNA，circ_0070934 的表达。探讨了 circ_0070934 在皮肤鳞状细胞癌（CSCC）中的生物学作用和机制。通过 qRT-PCR 评估 circ_0070934 在 CSCC 组织和细胞系中的表达。通过体外实验检测 circ_0070934 的缺失功能和获得功能对细胞增殖、凋亡、迁移和侵袭的影响。此外，通过在线数据库和荧光素酶报告基因实验预测 circ_0070934 的潜在分子机制。CSCC 样本和细胞系中检测到 circ_0070934 的异常过表达。circ_0070934 敲低后观察到细胞增殖、侵袭、迁移抑制和凋亡增加。circ_0070934 过表达细胞则观察到相反的效果。circ_0070934 的表达与 CSCC 中 miR-1238 和 miR-1247-5p 的表达呈负相关，荧光素酶报告实验验证了 circ_0070934 与 miR-1238/miR-1247-5p 的结合能力。拯救实验进一步证实了 circ_0070934 的致癌作用归因于其对 miR-1238 和 miR-1247-5p 的抑制作用。总之，我们的研究结果表明，circ_0070934 通过作为 CSCC 中的致癌 circRNA 与肿瘤侵袭性相关。
